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dc.contributor.authorMcGown, Alan T
dc.contributor.authorWard, Timothy H
dc.contributor.authorFox, Brian W
dc.date.accessioned2015-01-08T10:56:06Z
dc.date.available2015-01-08T10:56:06Z
dc.date.issued1983
dc.identifier.citationComparative studies of the uptake of daunorubicin in sensitive and resistant P388 cell lines by flow cytometry and biochemical extraction procedures. 1983, 11 (2):113-6 Cancer Chemother Pharmacolen
dc.identifier.issn0344-5704
dc.identifier.pmid6627597
dc.identifier.urihttp://hdl.handle.net/10541/337921
dc.description.abstractThe intracellular accumulation of daunorubicin as determined by flow cytometry correlates well with that as determined by extraction of the drug from cell homogenates. Two P388 mouse leukaemia cell lines showing differential sensitivity to the drug have been used to investigate the transport changes associated with resistance. Resistance to daunorubicin in these cell lines occurs through an alteration in the intracellular accumulation of the drug, resulting from the increased efflux of the anthracycline from the resistant cells. The effect of temperature, drug concentration, pH, and metabolic inhibitors on this process have been investigated. Uptake by a carrier-mediated process of the un-ionised form of the drug (pK = 8.25), coupled with an energy-dependent efflux process, is proposed as the mechanism of cellular accumulation in the case of the resistant cell line.
dc.language.isoenen
dc.rightsArchived with thanks to Cancer chemotherapy and pharmacologyen
dc.subject.meshAnimals
dc.subject.meshCell Line
dc.subject.meshDaunorubicin
dc.subject.meshDinitrophenols
dc.subject.meshDrug Resistance
dc.subject.meshFlow Cytometry
dc.subject.meshLeukemia P388
dc.subject.meshLeukemia, Experimental
dc.subject.meshMice
dc.subject.meshTemperature
dc.titleComparative studies of the uptake of daunorubicin in sensitive and resistant P388 cell lines by flow cytometry and biochemical extraction procedures.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalCancer Chemotherapy and Pharmacologyen
html.description.abstractThe intracellular accumulation of daunorubicin as determined by flow cytometry correlates well with that as determined by extraction of the drug from cell homogenates. Two P388 mouse leukaemia cell lines showing differential sensitivity to the drug have been used to investigate the transport changes associated with resistance. Resistance to daunorubicin in these cell lines occurs through an alteration in the intracellular accumulation of the drug, resulting from the increased efflux of the anthracycline from the resistant cells. The effect of temperature, drug concentration, pH, and metabolic inhibitors on this process have been investigated. Uptake by a carrier-mediated process of the un-ionised form of the drug (pK = 8.25), coupled with an energy-dependent efflux process, is proposed as the mechanism of cellular accumulation in the case of the resistant cell line.


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