Progesterone receptor measurement by isoelectric focusing: a potential microassay.
| dc.contributor.author | Harland, R N | |
| dc.contributor.author | Hayward, Elizabeth | |
| dc.contributor.author | Barnes, Diana M | |
| dc.date.accessioned | 2015-01-08T10:44:51Z | |
| dc.date.available | 2015-01-08T10:44:51Z | |
| dc.date.issued | 1983-09-30 | |
| dc.identifier.citation | Progesterone receptor measurement by isoelectric focusing: a potential microassay. 1983, 133 (2):159-68 Clin Chim Acta | en |
| dc.identifier.issn | 0009-8981 | |
| dc.identifier.pmid | 6194919 | |
| dc.identifier.uri | http://hdl.handle.net/10541/337916 | |
| dc.description.abstract | Sixty-two selected breast cancers were used to compare the conventional dextran-coated charcoal (DCC) assay with a new method of separating progesterone receptor by isoelectric focusing in flat beds of agarose gel. Ninety assays were performed. Isoelectric focusing indicated correctly the presence of receptor in 92% and the absence of receptor in 86% of assays, when compared with the DCC assay. The relationship between the results of the two methods was linear. Isoelectric focusing underestimated receptor to a variable extent, finding relatively less receptor at higher absolute levels of binding than at lower levels. The lower limit of sensitivity of isoelectric focusing was 30 fmol/ml cytosol. The protein concentrations of cytosols prepared from 46 needle biopsy samples (mean weight 25 mg) ranged from 0.5 to 30 g/l (median 4 g/l, 10th percentile 0.75 g/l). Isoelectric focusing is a satisfactory method of progesterone receptor measurement and can be applied to samples too small for conventional techniques. | |
| dc.language.iso | en | en |
| dc.rights | Archived with thanks to Clinica chimica acta; international journal of clinical chemistry | en |
| dc.subject.mesh | Biopsy, Needle | |
| dc.subject.mesh | Breast Neoplasms | |
| dc.subject.mesh | Charcoal | |
| dc.subject.mesh | Cytosol | |
| dc.subject.mesh | Dextrans | |
| dc.subject.mesh | Humans | |
| dc.subject.mesh | Isoelectric Focusing | |
| dc.subject.mesh | Microchemistry | |
| dc.subject.mesh | Receptors, Progesterone | |
| dc.title | Progesterone receptor measurement by isoelectric focusing: a potential microassay. | en |
| dc.type | Article | en |
| dc.contributor.department | University Department of Surgery, Withington Hospital, Manchester, UK | en |
| dc.identifier.journal | Clinica Chimica Acta | en |
| html.description.abstract | Sixty-two selected breast cancers were used to compare the conventional dextran-coated charcoal (DCC) assay with a new method of separating progesterone receptor by isoelectric focusing in flat beds of agarose gel. Ninety assays were performed. Isoelectric focusing indicated correctly the presence of receptor in 92% and the absence of receptor in 86% of assays, when compared with the DCC assay. The relationship between the results of the two methods was linear. Isoelectric focusing underestimated receptor to a variable extent, finding relatively less receptor at higher absolute levels of binding than at lower levels. The lower limit of sensitivity of isoelectric focusing was 30 fmol/ml cytosol. The protein concentrations of cytosols prepared from 46 needle biopsy samples (mean weight 25 mg) ranged from 0.5 to 30 g/l (median 4 g/l, 10th percentile 0.75 g/l). Isoelectric focusing is a satisfactory method of progesterone receptor measurement and can be applied to samples too small for conventional techniques. |