Show simple item record

dc.contributor.authorSchor, Seth L
dc.contributor.authorAllen, Terence D
dc.contributor.authorWinn, B
dc.date.accessioned2015-01-08T10:28:33Z
dc.date.available2015-01-08T10:28:33Z
dc.date.issued1983-04
dc.identifier.citationLymphocyte migration into three-dimensional collagen matrices: a quantitative study. 1983, 96 (4):1089-96 J Cell Biolen
dc.identifier.issn0021-9525
dc.identifier.pmid6833393
dc.identifier.urihttp://hdl.handle.net/10541/337912
dc.description.abstractLymphocytes have been plated onto the surface of three-dimensional gels of native collagen fibers, and their distribution throughout the three-dimensional collagen matrix has been determined in a quantitative fashion at various times thereafter. Information regarding the total number of applied cells may be obtained by this means. Lymphocyte penetration into the collagen gel does not appear to involve the expression of collagenolytic activity, nor does it require the presence of serum. Analysis of the kinetics of lymphocyte penetration into the gel matrix indicates that lymphocytes are migrating in a "random-walk" fashion. Our objective has been to establish a model system for studying the cell-matrix and cell-cell interactions which influence the pattern of lymphocyte recirculation in vivo and the results presented here are discussed in this context.
dc.language.isoenen
dc.rightsArchived with thanks to The Journal of cell biologyen
dc.subject.meshCell Adhesion
dc.subject.meshCell Communication
dc.subject.meshCell Movement
dc.subject.meshCollagen
dc.subject.meshHumans
dc.subject.meshLymphocytes
dc.subject.meshMicroscopy, Electron, Scanning
dc.titleLymphocyte migration into three-dimensional collagen matrices: a quantitative study.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Medical Oncology, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalJournal of Cell Biologyen
html.description.abstractLymphocytes have been plated onto the surface of three-dimensional gels of native collagen fibers, and their distribution throughout the three-dimensional collagen matrix has been determined in a quantitative fashion at various times thereafter. Information regarding the total number of applied cells may be obtained by this means. Lymphocyte penetration into the collagen gel does not appear to involve the expression of collagenolytic activity, nor does it require the presence of serum. Analysis of the kinetics of lymphocyte penetration into the gel matrix indicates that lymphocytes are migrating in a "random-walk" fashion. Our objective has been to establish a model system for studying the cell-matrix and cell-cell interactions which influence the pattern of lymphocyte recirculation in vivo and the results presented here are discussed in this context.


This item appears in the following Collection(s)

Show simple item record