Lymphocyte migration into three-dimensional collagen matrices: a quantitative study.
dc.contributor.author | Schor, Seth L | |
dc.contributor.author | Allen, Terence D | |
dc.contributor.author | Winn, B | |
dc.date.accessioned | 2015-01-08T10:28:33Z | |
dc.date.available | 2015-01-08T10:28:33Z | |
dc.date.issued | 1983-04 | |
dc.identifier.citation | Lymphocyte migration into three-dimensional collagen matrices: a quantitative study. 1983, 96 (4):1089-96 J Cell Biol | en |
dc.identifier.issn | 0021-9525 | |
dc.identifier.pmid | 6833393 | |
dc.identifier.uri | http://hdl.handle.net/10541/337912 | |
dc.description.abstract | Lymphocytes have been plated onto the surface of three-dimensional gels of native collagen fibers, and their distribution throughout the three-dimensional collagen matrix has been determined in a quantitative fashion at various times thereafter. Information regarding the total number of applied cells may be obtained by this means. Lymphocyte penetration into the collagen gel does not appear to involve the expression of collagenolytic activity, nor does it require the presence of serum. Analysis of the kinetics of lymphocyte penetration into the gel matrix indicates that lymphocytes are migrating in a "random-walk" fashion. Our objective has been to establish a model system for studying the cell-matrix and cell-cell interactions which influence the pattern of lymphocyte recirculation in vivo and the results presented here are discussed in this context. | |
dc.language.iso | en | en |
dc.rights | Archived with thanks to The Journal of cell biology | en |
dc.subject.mesh | Cell Adhesion | |
dc.subject.mesh | Cell Communication | |
dc.subject.mesh | Cell Movement | |
dc.subject.mesh | Collagen | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Lymphocytes | |
dc.subject.mesh | Microscopy, Electron, Scanning | |
dc.title | Lymphocyte migration into three-dimensional collagen matrices: a quantitative study. | en |
dc.type | Article | en |
dc.contributor.department | CRC Department of Medical Oncology, Christie Hospital and Holt Radium Institute, Manchester M20 9BX | en |
dc.identifier.journal | Journal of Cell Biology | en |
html.description.abstract | Lymphocytes have been plated onto the surface of three-dimensional gels of native collagen fibers, and their distribution throughout the three-dimensional collagen matrix has been determined in a quantitative fashion at various times thereafter. Information regarding the total number of applied cells may be obtained by this means. Lymphocyte penetration into the collagen gel does not appear to involve the expression of collagenolytic activity, nor does it require the presence of serum. Analysis of the kinetics of lymphocyte penetration into the gel matrix indicates that lymphocytes are migrating in a "random-walk" fashion. Our objective has been to establish a model system for studying the cell-matrix and cell-cell interactions which influence the pattern of lymphocyte recirculation in vivo and the results presented here are discussed in this context. |