Variance in resistance to natural and antibody-dependent cellular cytotoxicity and to complement-mediated lysis among K562 lines.
dc.contributor.author | Kimber, Ian | |
dc.contributor.author | Moore, Michael | |
dc.contributor.author | Roberts, Kevan | |
dc.date.accessioned | 2015-01-02T15:22:28Z | |
dc.date.available | 2015-01-02T15:22:28Z | |
dc.date.issued | 1983-08-15 | |
dc.identifier.citation | Variance in resistance to natural and antibody-dependent cellular cytotoxicity and to complement-mediated lysis among K562 lines. 1983, 32 (2):219-25 Int J Cancer | en |
dc.identifier.issn | 0020-7136 | |
dc.identifier.pmid | 6575968 | |
dc.identifier.uri | http://hdl.handle.net/10541/337760 | |
dc.description.abstract | Isolation of sub-populations of the NK-sensitive erythro-leukaemic cell line K562 by limiting dilution techniques has revealed marked clonal variation in susceptibility to natural cytotoxicity. Detailed examination of two such lines (E10/P2 and F9/P2) which differed significantly in their susceptibility to both native and activated NK-cell-mediated lysis revealed that their differences were stable and independent of culture conditions. The resistant (F9/P2) and sensitive (E10/P2) lines had comparable cold-inhibitory and effector-cell adsorption capacities, indicating that differential susceptibility was not attributable to variable expression of NK target structures. F9/P2 was also less susceptible to antibody-dependent cellular cytotoxicity (ADCC) and complement-mediated lysis, indicating the existence of variants with a generalized capacity to resist several immunolytic processes. | |
dc.language.iso | en | en |
dc.rights | Archived with thanks to International journal of cancer. Journal international du cancer | en |
dc.subject.mesh | Adsorption | |
dc.subject.mesh | Antibody-Dependent Cell Cytotoxicity | |
dc.subject.mesh | Cell Line | |
dc.subject.mesh | Cells, Cultured | |
dc.subject.mesh | Complement System Proteins | |
dc.subject.mesh | Cytotoxicity, Immunologic | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Immunity, Innate | |
dc.subject.mesh | Killer Cells, Natural | |
dc.subject.mesh | Leukemia, Erythroblastic, Acute | |
dc.title | Variance in resistance to natural and antibody-dependent cellular cytotoxicity and to complement-mediated lysis among K562 lines. | en |
dc.type | Article | en |
dc.contributor.department | Department of Immunology,Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX | en |
dc.identifier.journal | International Journal of Cancer | en |
html.description.abstract | Isolation of sub-populations of the NK-sensitive erythro-leukaemic cell line K562 by limiting dilution techniques has revealed marked clonal variation in susceptibility to natural cytotoxicity. Detailed examination of two such lines (E10/P2 and F9/P2) which differed significantly in their susceptibility to both native and activated NK-cell-mediated lysis revealed that their differences were stable and independent of culture conditions. The resistant (F9/P2) and sensitive (E10/P2) lines had comparable cold-inhibitory and effector-cell adsorption capacities, indicating that differential susceptibility was not attributable to variable expression of NK target structures. F9/P2 was also less susceptible to antibody-dependent cellular cytotoxicity (ADCC) and complement-mediated lysis, indicating the existence of variants with a generalized capacity to resist several immunolytic processes. |