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dc.contributor.authorKimber, Ianen
dc.contributor.authorMoore, Michaelen
dc.contributor.authorRoberts, Kevanen
dc.date.accessioned2015-01-02T15:22:28Z
dc.date.available2015-01-02T15:22:28Z
dc.date.issued1983-08-15
dc.identifier.citationVariance in resistance to natural and antibody-dependent cellular cytotoxicity and to complement-mediated lysis among K562 lines. 1983, 32 (2):219-25 Int J Canceren
dc.identifier.issn0020-7136
dc.identifier.pmid6575968
dc.identifier.urihttp://hdl.handle.net/10541/337760
dc.description.abstractIsolation of sub-populations of the NK-sensitive erythro-leukaemic cell line K562 by limiting dilution techniques has revealed marked clonal variation in susceptibility to natural cytotoxicity. Detailed examination of two such lines (E10/P2 and F9/P2) which differed significantly in their susceptibility to both native and activated NK-cell-mediated lysis revealed that their differences were stable and independent of culture conditions. The resistant (F9/P2) and sensitive (E10/P2) lines had comparable cold-inhibitory and effector-cell adsorption capacities, indicating that differential susceptibility was not attributable to variable expression of NK target structures. F9/P2 was also less susceptible to antibody-dependent cellular cytotoxicity (ADCC) and complement-mediated lysis, indicating the existence of variants with a generalized capacity to resist several immunolytic processes.
dc.language.isoenen
dc.rightsArchived with thanks to International journal of cancer. Journal international du canceren
dc.subject.meshAdsorption
dc.subject.meshAntibody-Dependent Cell Cytotoxicity
dc.subject.meshCell Line
dc.subject.meshCells, Cultured
dc.subject.meshComplement System Proteins
dc.subject.meshCytotoxicity, Immunologic
dc.subject.meshHumans
dc.subject.meshImmunity, Innate
dc.subject.meshKiller Cells, Natural
dc.subject.meshLeukemia, Erythroblastic, Acute
dc.titleVariance in resistance to natural and antibody-dependent cellular cytotoxicity and to complement-mediated lysis among K562 lines.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology,Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalInternational Journal of Canceren
html.description.abstractIsolation of sub-populations of the NK-sensitive erythro-leukaemic cell line K562 by limiting dilution techniques has revealed marked clonal variation in susceptibility to natural cytotoxicity. Detailed examination of two such lines (E10/P2 and F9/P2) which differed significantly in their susceptibility to both native and activated NK-cell-mediated lysis revealed that their differences were stable and independent of culture conditions. The resistant (F9/P2) and sensitive (E10/P2) lines had comparable cold-inhibitory and effector-cell adsorption capacities, indicating that differential susceptibility was not attributable to variable expression of NK target structures. F9/P2 was also less susceptible to antibody-dependent cellular cytotoxicity (ADCC) and complement-mediated lysis, indicating the existence of variants with a generalized capacity to resist several immunolytic processes.


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