AffiliationDepartment of Ultrastructure, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX
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AbstractFloating collagen gel cultures containing human foreskin fibroblasts have been observed to undergo a rapid contraction process. The initial rate of contraction (i.e., within the first 2 hr) was observed to be a linear function of cell number within the concentration range of 10(5)-10(6) cells/gel. Observation of thick, deresined sections of such contracting gels in the SEM, as well as observation of thin sections in the TEM, suggest that the fibroblasts exert a tension upon the surrounding collagen fibers. These observations further indicate that the fibroblasts migrate from the interior regions of the gel matrix and eventually form a monolayer of cells encapsulating the contracted collagen disc. These observations are discussed in terms of the possible mechanisms involved in gel contraction.
CitationThe contraction of collagen matrices by dermal fibroblasts. 1983, 83 (2):205-19 J Ultrastruct Res
JournalJournal of Ultrastructure Research
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