AffiliationPaterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester M20 9BX
MetadataShow full item record
Abstract1. The lipid fluidity of three major rat liver plasma-membrane subfractions, as well as Golgi apparatus and endocytic fractions, was assessed with a fatty acid spin probe by using e.s.r. techniques. 2. The sinusoidal (blood-facing) plasma-membrane subfraction was the most fluid of the three plasma-membrane regions. Fractions originating from the bile-canalicular and contiguous (lateral) regions were most rigid. Endocytic fractions isolated (endosomes and diacytosomes) were of a similar fluidity to fractions originating from the sinusoidal plasma-membrane region. By far the most fluid fractions examined were derived from the Golgi-apparatus complex. 3. The three plasma-membrane subfractions each showed a different response to the bilayer-fluidizing effect of benzyl alcohol. 4. Arrhenius-type plots of the order parameter S and outer hyperfine splitting, 2T( parallel), identified lipid-phase separations in the plasma-membrane subfractions.
CitationThe lipid fluidity of rat liver membrane subfractions. 1983, 214 (3):851-4 Biochem J
- The lipid composition of plasma membrane subfractions originating from the three major functional domains of the rat hepatocyte cell surface.
- Authors: Kremmer T, Wisher MH, Evans WH
- Issue date: 1976 Dec 14
- Vitamin E supplement improves erythrocyte membrane fluidity of thalassemia: an ESR spin labeling study.
- Authors: Sutipornpalangkul W, Morales NP, Unchern S, Sanvarinda Y, Chantharaksri U, Fucharoen S
- Issue date: 2012 Jan
- Biogenesis of hepatocyte plasma-membrane domains. Incorporation of (3H)fucose into plasma-membrane and golgi-apparatus glycoproteins.
- Authors: Evans WH, Flint NA, Vischer P
- Issue date: 1980 Dec 15
- A two-dimensional electrophoretic analysis of the proteins and glycoproteins of liver plasma membrane domains and endosomes. Implications for endocytosis and transcytosis.
- Authors: Enrich C, Tabona P, Evans WH
- Issue date: 1990 Oct 1