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dc.contributor.authorBakács, T
dc.contributor.authorRingwald, G
dc.contributor.authorTótpál, K
dc.contributor.authorKimber, I
dc.contributor.authorMoore, Michael
dc.date.accessioned2015-01-02T15:19:21Z
dc.date.available2015-01-02T15:19:21Z
dc.date.issued1983-08
dc.identifier.citationThe relationship between antibody concentration and target cell number in the induction of K cell-mediated haemolysis: evidence for asymmetry among reactants. 1983, 11 (4):189-95 J Clin Lab Immunolen
dc.identifier.issn0141-2760
dc.identifier.pmid6321739
dc.identifier.urihttp://hdl.handle.net/10541/337727
dc.description.abstractThe cytotoxic capacity of peripheral blood mononuclear cells for anti-D sensitized human O Rh(D) + ve (R1R2) erythrocytes was measured under conditions where antibody concentrations and target cell number were varied with respect to each other. It was observed that where sensitization was limiting, due either to target cell excess or increased antibody dilution, the relationship between lysis and antibody concentration was non-linear, and that the end-point dilution of anti-D at which measurable cytotoxicity occurred was dependent upon the source or concentration of effector cells. The asymmetrical relationship between cytotoxic activity and the degree of sensitization suggests that, in this system at least, there is extensive heterogeneity either among target erythrocytes in terms of their degree of sensitization and consequent susceptibility to lysis, and/or among effector cells in terms of their sensitization requirements for induction of cytotoxicity.
dc.language.isoenen
dc.rightsArchived with thanks to Journal of clinical & laboratory immunologyen
dc.subject.meshAntibody-Dependent Cell Cytotoxicity
dc.subject.meshCytotoxicity Tests, Immunologic
dc.subject.meshDose-Response Relationship, Immunologic
dc.subject.meshFemale
dc.subject.meshHemolysis
dc.subject.meshHumans
dc.subject.meshImmunization
dc.subject.meshImmunoglobulins
dc.subject.meshKiller Cells, Natural
dc.subject.meshPregnancy
dc.subject.meshRh-Hr Blood-Group System
dc.subject.meshRho(D) Immune Globulin
dc.titleThe relationship between antibody concentration and target cell number in the induction of K cell-mediated haemolysis: evidence for asymmetry among reactants.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalJournal of Clinical & Laboratory Immunologyen
html.description.abstractThe cytotoxic capacity of peripheral blood mononuclear cells for anti-D sensitized human O Rh(D) + ve (R1R2) erythrocytes was measured under conditions where antibody concentrations and target cell number were varied with respect to each other. It was observed that where sensitization was limiting, due either to target cell excess or increased antibody dilution, the relationship between lysis and antibody concentration was non-linear, and that the end-point dilution of anti-D at which measurable cytotoxicity occurred was dependent upon the source or concentration of effector cells. The asymmetrical relationship between cytotoxic activity and the degree of sensitization suggests that, in this system at least, there is extensive heterogeneity either among target erythrocytes in terms of their degree of sensitization and consequent susceptibility to lysis, and/or among effector cells in terms of their sensitization requirements for induction of cytotoxicity.


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