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dc.contributor.authorHall, Janet A
dc.contributor.authorSaffhill, Roy
dc.date.accessioned2015-01-02T15:14:17Z
dc.date.available2015-01-02T15:14:17Z
dc.date.issued1983-06-25
dc.identifier.citationThe incorporation of O6-methyldeoxyguanosine and O4-methyldeoxythymidine monophosphates into DNA by DNA polymerases I and alpha. 1983, 11 (12):4185-93 Nucleic Acids Resen
dc.identifier.issn0305-1048
dc.identifier.pmid6866769
dc.identifier.urihttp://hdl.handle.net/10541/337726
dc.description.abstractThe modified nucleoside 5'-triphosphates O6-MedGTP ad O4-MedTTP have been synthesised and their acceptability as DNA-precursors investigated using DNA polymerases I and alpha in an in vitro assay. O6-MedGMP is only incorporated into newly-synthesized DNA-like material in the presence of templates containing thymine bases. Similarly O4-MedTMP is only incorporated in the presence of templates containing guanine bases. The results confirm the promutagenic nature and base-pairing properties of O6-MeG ad O4-MeT.
dc.language.isoenen
dc.rightsArchived with thanks to Nucleic acids researchen
dc.subject.meshDNA Polymerase I
dc.subject.meshDNA Polymerase II
dc.subject.meshDNA Replication
dc.subject.meshDNA-Directed DNA Polymerase
dc.subject.meshDeoxyguanine Nucleotides
dc.subject.meshThymidine Monophosphate
dc.subject.meshThymine Nucleotides
dc.titleThe incorporation of O6-methyldeoxyguanosine and O4-methyldeoxythymidine monophosphates into DNA by DNA polymerases I and alpha.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalNucleic Acids Researchen
html.description.abstractThe modified nucleoside 5'-triphosphates O6-MedGTP ad O4-MedTTP have been synthesised and their acceptability as DNA-precursors investigated using DNA polymerases I and alpha in an in vitro assay. O6-MedGMP is only incorporated into newly-synthesized DNA-like material in the presence of templates containing thymine bases. Similarly O4-MedTMP is only incorporated in the presence of templates containing guanine bases. The results confirm the promutagenic nature and base-pairing properties of O6-MeG ad O4-MeT.


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