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dc.contributor.authorHendry, Jolyon H
dc.contributor.authorPotten, Christopher S
dc.contributor.authorRoberts, N P
dc.date.accessioned2015-01-02T15:01:19Z
dc.date.available2015-01-02T15:01:19Z
dc.date.issued1983-10
dc.identifier.citationThe gastrointestinal syndrome and mucosal clonogenic cells: relationships between target cell sensitivities, LD50 and cell survival, and their modification by antibiotics. 1983, 96 (1):100-12 Radiat Resen
dc.identifier.issn0033-7587
dc.identifier.pmid6353474
dc.identifier.urihttp://hdl.handle.net/10541/337724
dc.description.abstractThe sensitivity of the target cells responsible for the gastrointestinal syndrome in mice was deduced from the steepness of the dose-survival curve for mice assessed on Day 7 after irradiation. The D0 value was 1.25 +/- 0.22 Gy, virtually identical to the value of 1.23 +/- 0.08 measured for microcolony-forming cells (clonogens) over about the same range of dose in concurrent experiments. The survival of clonogens was similar when assayed in mice surviving to Days 3, 4, or 5, but clonogenic sensitivity was lower when assessed on Day 7. This was shown at one dose to be due largely to a selection of mice with high colony counts with only a small contribution from crypt budding. The LD50 for mice corresponded to a surviving fraction of crypts of about 0.35. An injection of 5 mg streptomycin sulphate ip daily for 5 days after irradiation increased the latent period by about 1 day, increased the LD50 by about 1.4 Gy, but did not significantly change the survival of clonogens. These studies are the first to test and satisfy the interpretation of a dose-response curve for animal survival in terms of "target cell" survival, where measurements of both are made over a similar range of dose in concurrent experiments.
dc.language.isoenen
dc.rightsArchived with thanks to Radiation researchen
dc.subject.meshAnimals
dc.subject.meshCell Survival
dc.subject.meshClone Cells
dc.subject.meshDigestive System
dc.subject.meshDose-Response Relationship, Radiation
dc.subject.meshIntestinal Mucosa
dc.subject.meshLethal Dose 50
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMice, Inbred Strains
dc.subject.meshRadiation Injuries, Experimental
dc.subject.meshStreptomycin
dc.subject.meshSyndrome
dc.subject.meshTime Factors
dc.subject.meshWhole-Body Irradiation
dc.titleThe gastrointestinal syndrome and mucosal clonogenic cells: relationships between target cell sensitivities, LD50 and cell survival, and their modification by antibiotics.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalRadiation Researchen
html.description.abstractThe sensitivity of the target cells responsible for the gastrointestinal syndrome in mice was deduced from the steepness of the dose-survival curve for mice assessed on Day 7 after irradiation. The D0 value was 1.25 +/- 0.22 Gy, virtually identical to the value of 1.23 +/- 0.08 measured for microcolony-forming cells (clonogens) over about the same range of dose in concurrent experiments. The survival of clonogens was similar when assayed in mice surviving to Days 3, 4, or 5, but clonogenic sensitivity was lower when assessed on Day 7. This was shown at one dose to be due largely to a selection of mice with high colony counts with only a small contribution from crypt budding. The LD50 for mice corresponded to a surviving fraction of crypts of about 0.35. An injection of 5 mg streptomycin sulphate ip daily for 5 days after irradiation increased the latent period by about 1 day, increased the LD50 by about 1.4 Gy, but did not significantly change the survival of clonogens. These studies are the first to test and satisfy the interpretation of a dose-response curve for animal survival in terms of "target cell" survival, where measurements of both are made over a similar range of dose in concurrent experiments.


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