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dc.contributor.authorAllen, Terence D
dc.date.accessioned2014-12-23T15:00:30Z
dc.date.available2014-12-23T15:00:30Z
dc.date.issued1983
dc.identifier.citationThe application of scanning electron microscopy to cells in culture: selected methodologies. 1983 (Pt 4):1963-72 Scan Electron Microscen
dc.identifier.issn0586-5581
dc.identifier.pmid6422539
dc.identifier.urihttp://hdl.handle.net/10541/337611
dc.description.abstractCell culture is one of the most widely used techniques in modern biology. As a result, morphology of culture systems at the ultrastructural level is also becoming more widespread. Whilst offering an optimum in terms of fixation accessibility, cells in tissue culture can present problems in terms of handling for scanning electron microscopy, particularly when they are grown on, or in semi-solid media, or on gels e.g. collagen. The examination of cultured cell suspensions or mixed cell populations may also require careful handling to produce meaningful results in terms of surface morphology and cellular interaction. Cells specifically cultured on carbon films can also be used for information of internal cell structure as well as surface topography, often simultaneously, if a suitable imaging system is available. This communication reviews a wide spectrum of experimental manipulations of cultured material to provide an introduction to the gathering of morphological information from material in tissue culture.
dc.language.isoenen
dc.rightsArchived with thanks to Scanning electron microscopyen
dc.subject.meshAnimals
dc.subject.meshCell Line
dc.subject.meshCells, Cultured
dc.subject.meshCollagen
dc.subject.meshCricetinae
dc.subject.meshCricetulus
dc.subject.meshCulture Media
dc.subject.meshFemale
dc.subject.meshGlutaral
dc.subject.meshLiver
dc.subject.meshLiver Neoplasms, Experimental
dc.subject.meshMicroscopy, Electron
dc.subject.meshMicroscopy, Electron, Scanning
dc.subject.meshMitochondria, Liver
dc.subject.meshOvary
dc.subject.meshRats
dc.titleThe application of scanning electron microscopy to cells in culture: selected methodologies.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalScanning Electron Microscopyen
html.description.abstractCell culture is one of the most widely used techniques in modern biology. As a result, morphology of culture systems at the ultrastructural level is also becoming more widespread. Whilst offering an optimum in terms of fixation accessibility, cells in tissue culture can present problems in terms of handling for scanning electron microscopy, particularly when they are grown on, or in semi-solid media, or on gels e.g. collagen. The examination of cultured cell suspensions or mixed cell populations may also require careful handling to produce meaningful results in terms of surface morphology and cellular interaction. Cells specifically cultured on carbon films can also be used for information of internal cell structure as well as surface topography, often simultaneously, if a suitable imaging system is available. This communication reviews a wide spectrum of experimental manipulations of cultured material to provide an introduction to the gathering of morphological information from material in tissue culture.


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