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dc.contributor.authorGraham, Annika
dc.contributor.authorFox, Margaret
dc.date.accessioned2014-12-23T14:51:37Z
dc.date.available2014-12-23T14:51:37Z
dc.date.issued1983
dc.identifier.citationThe role of suppression of DNA synthesis and inhibition of cell cycle progression in cellular sensitivity to alkylation damage. 1983, 4 (3):269-74 Carcinogenesisen
dc.identifier.issn0143-3334
dc.identifier.pmid6831633
dc.identifier.doi10.1093/carcin/4.3/269
dc.identifier.urihttp://hdl.handle.net/10541/337596
dc.description.abstractA u.v. sensitive Chinese hamster cell line V79/79 has been shown to be also more sensitive to methyl methanesulphonate (MMS) and nitrogen mustard (HN2) exposure than wild-type V79 cells. A comparison of the effects of the two alkylating agents on DNA synthesis measured by [3H]thymidine (TdR) incorporation into whole cells and by alkaline sucrose gradient sedimentation 14C-labelled template and of pulse labelled DNA revealed no significant differences between the responses of the two cell lines. The effects of a range of doses of both drugs on the rate of progress through the cell cycle was compared using cytofluorimetry. The more sensitive V79/79 cells failed to show a significant delay in progress through the cell cycle even at the highest doses tested (0.2 microM HN2 and 2.0 mM MMS). In contrast, V79 cells showed a marked S phase delay in response to both HN2 and MMS exposure. The possible relationships between failure to delay cell cycle progression, and cellular sensitivity are discussed.
dc.language.isoenen
dc.rightsArchived with thanks to Carcinogenesisen
dc.subject.meshAnimals
dc.subject.meshCell Cycle
dc.subject.meshCell Line
dc.subject.meshCricetinae
dc.subject.meshCricetulus
dc.subject.meshDNA
dc.subject.meshDNA Replication
dc.subject.meshGenetic Variation
dc.subject.meshKinetics
dc.subject.meshLung
dc.subject.meshMechlorethamine
dc.subject.meshMethyl Methanesulfonate
dc.subject.meshMolecular Weight
dc.titleThe role of suppression of DNA synthesis and inhibition of cell cycle progression in cellular sensitivity to alkylation damage.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester M20 9BXen
dc.identifier.journalCarcinogenesisen
html.description.abstractA u.v. sensitive Chinese hamster cell line V79/79 has been shown to be also more sensitive to methyl methanesulphonate (MMS) and nitrogen mustard (HN2) exposure than wild-type V79 cells. A comparison of the effects of the two alkylating agents on DNA synthesis measured by [3H]thymidine (TdR) incorporation into whole cells and by alkaline sucrose gradient sedimentation 14C-labelled template and of pulse labelled DNA revealed no significant differences between the responses of the two cell lines. The effects of a range of doses of both drugs on the rate of progress through the cell cycle was compared using cytofluorimetry. The more sensitive V79/79 cells failed to show a significant delay in progress through the cell cycle even at the highest doses tested (0.2 microM HN2 and 2.0 mM MMS). In contrast, V79 cells showed a marked S phase delay in response to both HN2 and MMS exposure. The possible relationships between failure to delay cell cycle progression, and cellular sensitivity are discussed.


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