The role of suppression of DNA synthesis and inhibition of cell cycle progression in cellular sensitivity to alkylation damage.
Affiliation
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester M20 9BXIssue Date
1983
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A u.v. sensitive Chinese hamster cell line V79/79 has been shown to be also more sensitive to methyl methanesulphonate (MMS) and nitrogen mustard (HN2) exposure than wild-type V79 cells. A comparison of the effects of the two alkylating agents on DNA synthesis measured by [3H]thymidine (TdR) incorporation into whole cells and by alkaline sucrose gradient sedimentation 14C-labelled template and of pulse labelled DNA revealed no significant differences between the responses of the two cell lines. The effects of a range of doses of both drugs on the rate of progress through the cell cycle was compared using cytofluorimetry. The more sensitive V79/79 cells failed to show a significant delay in progress through the cell cycle even at the highest doses tested (0.2 microM HN2 and 2.0 mM MMS). In contrast, V79 cells showed a marked S phase delay in response to both HN2 and MMS exposure. The possible relationships between failure to delay cell cycle progression, and cellular sensitivity are discussed.Citation
The role of suppression of DNA synthesis and inhibition of cell cycle progression in cellular sensitivity to alkylation damage. 1983, 4 (3):269-74 CarcinogenesisJournal
CarcinogenesisDOI
10.1093/carcin/4.3/269PubMed ID
6831633Type
ArticleLanguage
enISSN
0143-3334ae974a485f413a2113503eed53cd6c53
10.1093/carcin/4.3/269