Differential responses of nascent DNA synthesis and chain elongation in V79 and V79/79 cells exposed to u.v. light and chemical mutagens.
Affiliation
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXIssue Date
1983
Metadata
Show full item recordAbstract
DNA repair after u.v., N-methyl-N-nitrosourea (MNU) and ethylmethane sulphonate (EMS) in Chinese hamster V79 cells and the mutagen sensitive derivative V79/79 was investigated by measurement of five parameters: production of strand breaks in template DNA, incorporation of [3H]TdR, semi-conservative and repair synthesis, molecular weights of pulse labelled DNA after mutagen exposure (nascent synthesis) and molecular weights of DNA pulse labelled and chased after mutagen exposure (elongation and ligation). Equal template strand breakage was evident in both cell lines immediately after MNU and EMS exposure and by 4-5 h after MNU the extent of fragmentation was greater in V79/79 cells. After u.v. irradiation template fragmentation was evident in V79/79 but not in V79 cells, even though V79/79 cells failed to excise cyclobutane dimers and repair synthesis was demonstrable in V79 cells but not in V79/79 cells after exposure to all three mutagens. The rate of incorporation of [3H]TdR during semi-conservative DNA synthesis was inhibited equally in a dose dependent manner after u.v. and MNU exposure; incorporation by V79/79 cells was inhibited to a greater extent than by V79 cells after EMS exposure. Nascent DNA synthesis was suppressed more in V79/79 cells than in V79 cells after u.v. but to similar extents in both cell lines after MNU and EMS treatment. Pulse chase experiments indicated a lower rate of elongation of nascent DNA in V79/79 cells after MNU and u.v. exposure but little difference was detectable after EMS.Citation
Differential responses of nascent DNA synthesis and chain elongation in V79 and V79/79 cells exposed to u.v. light and chemical mutagens. 1983, 4 (3):261-8 CarcinogenesisJournal
CarcinogenesisDOI
10.1093/carcin/4.3.261PubMed ID
6831632Type
ArticleLanguage
enISSN
0143-3334ae974a485f413a2113503eed53cd6c53
10.1093/carcin/4.3.261
Scopus Count
Collections
Related articles
- Relative sensitivity of V79 and V79/79 cells to spontaneous and induced mutation to 6-thioguanine and ouabain resistance.
- Authors: Fox M, McMillan S, Durrant L, Boyle JM
- Issue date: 1982 Aug
- Nuclease sensitivity of repair-incorporated nucleotides in chromatin and nucleosome rearrangement in human cells damaged by methyl methanesulfonate and methylnitrosourea.
- Authors: Sidik K, Smerdon MJ
- Issue date: 1984 Feb
- Drosophila mutations at the mei-9 and mus(2)201 loci which block excision of thymine dimers also block induction of unscheduled DNA synthesis by methyl methanesulfonate, ethyl methanesulfonate, N-methyl-N-nitrosourea, UV light and X-rays.
- Authors: Dusenbery RL, McCormick SC, Smith PD
- Issue date: 1983 Aug
- Postreplication repair of alkylation damage to DNA of mammalian cells in culture.
- Authors: Fujiwara Y
- Issue date: 1975 Oct
- Differences between survival, mutagenicity and DNA replication in MMS- and MNU-treated V79 hamster cells.
- Authors: Slamenová D, Dusinská M, Bastlová T, Gábelová A
- Issue date: 1990 Jan