• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Incorporation and persistence of 5-bromodeoxyuridine in newborn rat tissue DNA.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Likhachev, A J
    Tomatis, L
    Margison, Geoffrey P
    Affiliation
    International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon Cedex 08 France
    Issue Date
    1983-08-15
    
    Metadata
    Show full item record
    Abstract
    One-day-old BDVI rats were given a mixture 5-[3H]bromodeoxyuridine (BrUdR) and [14C]thymidine (each at 20 mg/kg; 12.8 and 3.53 mCi/mmol, respectively) by intraperitoneal injection. DNA was isolated by a phenol procedure from various pooled tissues up to 21 days later and the levels of BrUdR and thymidine incorporation were determined after formic acid hydrolysis and Dowex-50 chromatography. Incorporation increased to a maximum at 12 h then decreased, the decrease of both products occurring in parallel in all tissues examined but differing in kinetics from tissue to tissue. After 12 h, the relative amounts of 3H and 14C showed no consistent or marked decreases and this suggests that BrUdR is not actively removed from DNA by a repair process under these experimental conditions. This was also the case after a much lower dose of these agents (9 micrograms/kg [3H]BrUdR 22.6 Ci/mmol; 1.3 mg/kg [14C]thymidine 53 mCi/mmol) when the 3H/14C ratios in DNA from various tissues were increased relative to the higher dose and showed only slight differences between 15 h and 21 days after administration.
    Citation
    Incorporation and persistence of 5-bromodeoxyuridine in newborn rat tissue DNA. 1983, 46 (1):31-8 Chem Biol Interact
    Journal
    Chemico-Biological Interactions
    URI
    http://hdl.handle.net/10541/337586
    PubMed ID
    6616707
    Type
    Article
    Language
    en
    ISSN
    0009-2797
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Altered histone-DNA interactions in rat liver chromatin containing 5-bromodeoxyuridine-substituted DNA.
    • Authors: Matthes E, Fenske H, Eichhorn I, Langen P, Lindigkeit R
    • Issue date: 1977 Oct
    • Incorporation of 5-bromodeoxyuridine into DNA in newborn rat tissues.
    • Authors: Margison GP, Likhachev AJ, Tomatis L
    • Issue date: 1980 Jun
    • Immunohistochemical detection of proliferating cells in vivo.
    • Authors: deFazio A, Leary JA, Hedley DW, Tattersall MH
    • Issue date: 1987 May
    • Effect of acute doses of 2-acetylaminofluorene on the capacity of rat liver to repair methylated purines in DNA in vivo and in vitro.
    • Authors: Cooper DP, O'Connor PJ, Margison GP
    • Issue date: 1982 Oct
    • Identification of linoleic and arachidonic acids as the factors in hyperlipemic blood that increase [3H]thymidine incorporation in hepatoma 7288CTC perfused in situ.
    • Authors: Sauer LA, Dauchy RT
    • Issue date: 1988 Jun 1
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.