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    Co-ordination of cell cycle, migration and stem cell-like activity in breast cancer.

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    Authors
    Lamb, Rebecca
    Lisanti, Michael P
    Clarke, Robert B
    Landberg, Göran
    Affiliation
    Breakthrough Breast Cancer Unit , Institute of Cancer Sciences, Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, UK.
    Issue Date
    2014-09-15
    
    Metadata
    Show full item record
    Abstract
    Migration, proliferation and stem cell-like activity are all key cellular characteristics which aid the formation and progression of breast cancer, in addition to involvement in treatment resistance. Many current therapies aim to target tumour proliferation, and although successful, mortality rates in breast cancer remain significant. Our main objectives were to investigate the relationship between proliferation, migration and stem cell-like activity in breast cancer. We used a panel of cell lines and primary human breast cancer samples to assess the relationship between migration, proliferation and stem cells. We performed live cell sorting according to cell cycle (Hoechst-33324) and in combination with stem-cell markers (CD44/CD24/ESA) followed by assessment of migration and stem cell activity (mammosphere formation). We identified an inverse relationship between proliferation and migration/stem cell-like activity. G0/1 cells showed increased migration and mammosphere formation. Furthermore we identified a subpopulation of low proliferative stem-like cells (CD44+/24lo/ESA+) with increased migration and mammosphere formation that are specifically inhibited by Dickkopf 1 (DKK1) and Dibenzazepine (DBZ) known stem-cell inhibitors. These data show the co-ordination of migration, proliferation and stem cell activity in breast cancer, and has identified a sub-population of stem-like cells, greatly adding to our understanding of the complex nature of stem cell biology.
    Citation
    Co-ordination of cell cycle, migration and stem cell-like activity in breast cancer. 2014, 5 (17):7833-42 Oncotarget
    Journal
    Oncotarget
    URI
    http://hdl.handle.net/10541/333863
    PubMed ID
    25277201
    Type
    Article
    Language
    en
    ISSN
    1949-2553
    Collections
    All Paterson Institute for Cancer Research

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