Transmission FT-IR chemical imaging on glass substrates: applications in infrared spectral histopathology.
Affiliation
Manchester Institute of Biotechnology, University of Manchester , 131 Princess Street, ManchesterIssue Date
2014-02-04
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Fourier transform-infrared (FT-IR) chemical imaging in transmission mode has traditionally been performed on expensive mid-IR transparent windows such as barium/calcium fluoride, which are more fragile than glass, making preparation in the histopathology laboratories more cumbersome. A solution is presented here by using cheap glass substrates for the FT-IR chemical imaging, which has a high-wavenumber transmission window allowing measurement of the C-H, N-H, and O-H stretches occurring at ca. 2500-3800 cm(-1). The "fingerprint" region of the IR spectrum occurring below 1800 cm(-1) is not obtainable; however, we demonstrate that a wealth of information is contained in the high wavenumber range using 71 patients on a breast tissue microarray (TMA) as a model for investigation. Importantly, we demonstrate that the tissue can be classified into four basic tissue cell types and that using just the epithelial cells, reasonable discrimination of normal and malignant tissue can be found.Citation
Transmission FT-IR chemical imaging on glass substrates: applications in infrared spectral histopathology. 2014, 86 (3):1648-53 Anal ChemJournal
Analytical ChemistryDOI
10.1021/ac403412nPubMed ID
24410403Type
ArticleLanguage
enISSN
1520-6882ae974a485f413a2113503eed53cd6c53
10.1021/ac403412n
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