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    Transmission FT-IR chemical imaging on glass substrates: applications in infrared spectral histopathology.

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    Authors
    Bassan, P
    Mellor, J
    Shapiro, J
    Williams, K
    Lisanti, Michael P
    Gardner, P
    Affiliation
    Manchester Institute of Biotechnology, University of Manchester , 131 Princess Street, Manchester
    Issue Date
    2014-02-04
    
    Metadata
    Show full item record
    Abstract
    Fourier transform-infrared (FT-IR) chemical imaging in transmission mode has traditionally been performed on expensive mid-IR transparent windows such as barium/calcium fluoride, which are more fragile than glass, making preparation in the histopathology laboratories more cumbersome. A solution is presented here by using cheap glass substrates for the FT-IR chemical imaging, which has a high-wavenumber transmission window allowing measurement of the C-H, N-H, and O-H stretches occurring at ca. 2500-3800 cm(-1). The "fingerprint" region of the IR spectrum occurring below 1800 cm(-1) is not obtainable; however, we demonstrate that a wealth of information is contained in the high wavenumber range using 71 patients on a breast tissue microarray (TMA) as a model for investigation. Importantly, we demonstrate that the tissue can be classified into four basic tissue cell types and that using just the epithelial cells, reasonable discrimination of normal and malignant tissue can be found.
    Citation
    Transmission FT-IR chemical imaging on glass substrates: applications in infrared spectral histopathology. 2014, 86 (3):1648-53 Anal Chem
    Journal
    Analytical Chemistry
    URI
    http://hdl.handle.net/10541/315923
    DOI
    10.1021/ac403412n
    PubMed ID
    24410403
    Type
    Article
    Language
    en
    ISSN
    1520-6882
    ae974a485f413a2113503eed53cd6c53
    10.1021/ac403412n
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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