Microfluidic channel-assisted screening of hematopoietic malignancies.
AffiliationManchester Interdisciplinary Biocentre, University of Manchester, Manchester, M1, UK
MetadataShow full item record
AbstractA simple microfluidic fluorescence in situ hybridization (FISH) device allowing accurate analysis of interphase nuclei in 1 hr in narrow channels is presented. Photolithography and fluorosilicic acid etching were used to fabricate microfluidic channels (referred to as FISHing lines) that allowed analysis of 10 samples on a glass microscope slide 0.2 µl of sample volume was used to fill a micro-channel, which resembled a 250-fold reduction compared to conventional FISH. FISH signals were comparable to conventional FISH, with 50-fold less probe consumption and 10-fold less time. Cells were immobilized in single file in channels just exceeding the diameter of the cells, and were used for minimal residual disease (MRD) analysis. To test the micro-channels for application in FISH, MRD was simulated by mixing K562 cells (an established chronic myeloid leukemia cell line) carrying the BCR/ABL fusion gene across 1:1 to 1:1,000 Jurkat cells (an established acute lymphoblastic leukemia cell line). The limit of detection was seen to be 1:100 cells and 1:1,000 cells for FISHing lines and conventional FISH, respectively; however, the conventional method seemed to over-score the presence of K562 cells. This may in part be attributed to FISHing lines practically eliminating the chance of duplicate screening of cells and hastened the time of screening, enhancing scoring of all cells within the channels. This was compared to 1 in 500 cells on the slide being analyzed with the conventional FISH.
CitationMicrofluidic channel-assisted screening of hematopoietic malignancies. 2014, 53 (3):255-63 Genes Chromosomes Cancer
JournalGenes, Chromosomes & Cancer
- Automated Duet spot counting system and manual technologist scoring using dual-fusion fluorescence in situ hybridization (D-FISH) strategy: comparison and application to FISH minimal residual disease testing in patients with chronic myeloid leukemia.
- Authors: Knudson RA, Shearer BM, Ketterling RP
- Issue date: 2007 May
- [Detection of abnormal numbers of chromosome 8 with interphase fluorescence in situ hybridization in hematologic malignancies].
- Authors: Wang HP, Li GX, Qiao ZH, Wang HW
- Issue date: 2004 Aug
- [The application of dual fusion interphase fluorescence in situ hybridization probe in detecting minimal residual disease in chronic myeloid leukemia].
- Authors: Du Q, Liu X, Song L, Meng F, Zhou S
- Issue date: 2002 Dec
- Miniaturized FISH for screening of onco-hematological malignancies.
- Authors: Zanardi A, Bandiera D, Bertolini F, Corsini CA, Gregato G, Milani P, Barborini E, Carbone R
- Issue date: 2010 Jul
- Clinical significance of dual color-dual fusion translocation fluorescence in situ hybridization in the detection of bcr/abl fusion gene.
- Authors: Wu B, Zhou S, Song L, Liu X
- Issue date: 2002 Jul