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    Microfluidic channel-assisted screening of hematopoietic malignancies.

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    Authors
    Mughal, F
    Baldock, S
    Karimiani, E
    Telford, Nicholas
    Goddard, N
    Day, P
    Affiliation
    Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, M1, UK
    Issue Date
    2014-03
    
    Metadata
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    Abstract
    A simple microfluidic fluorescence in situ hybridization (FISH) device allowing accurate analysis of interphase nuclei in 1 hr in narrow channels is presented. Photolithography and fluorosilicic acid etching were used to fabricate microfluidic channels (referred to as FISHing lines) that allowed analysis of 10 samples on a glass microscope slide 0.2 µl of sample volume was used to fill a micro-channel, which resembled a 250-fold reduction compared to conventional FISH. FISH signals were comparable to conventional FISH, with 50-fold less probe consumption and 10-fold less time. Cells were immobilized in single file in channels just exceeding the diameter of the cells, and were used for minimal residual disease (MRD) analysis. To test the micro-channels for application in FISH, MRD was simulated by mixing K562 cells (an established chronic myeloid leukemia cell line) carrying the BCR/ABL fusion gene across 1:1 to 1:1,000 Jurkat cells (an established acute lymphoblastic leukemia cell line). The limit of detection was seen to be 1:100 cells and 1:1,000 cells for FISHing lines and conventional FISH, respectively; however, the conventional method seemed to over-score the presence of K562 cells. This may in part be attributed to FISHing lines practically eliminating the chance of duplicate screening of cells and hastened the time of screening, enhancing scoring of all cells within the channels. This was compared to 1 in 500 cells on the slide being analyzed with the conventional FISH.
    Citation
    Microfluidic channel-assisted screening of hematopoietic malignancies. 2014, 53 (3):255-63 Genes Chromosomes Cancer
    Journal
    Genes, Chromosomes & Cancer
    URI
    http://hdl.handle.net/10541/314885
    DOI
    10.1002/gcc.22137
    PubMed ID
    24339206
    Type
    Article
    Language
    en
    ISSN
    1098-2264
    ae974a485f413a2113503eed53cd6c53
    10.1002/gcc.22137
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