Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy.
Abstract
FTIR chemical imaging has been demonstrated as a promising technique to construct automated systems to complement histopathological evaluation of biomedical tissue samples. The rapid chemical imaging of large areas of tissue has previously been a limiting factor in this application. Consequently, smaller areas of tissue have previously had to be sampled, possibly introducing sampling bias and potentially missing diagnostically important areas. In this report a high spatial resolution chemical image of a whole prostate cross section is shown comprising 66 million pixels. Each pixel represents an area 5.5 × 5.5 μm(2) of tissue and contains a full infrared spectrum providing a chemical fingerprint. The data acquisition time was 14 hours, thus showing that a clinical time frame of hours rather than days has been achieved.Citation
Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy. 2013, 138 (23):7066-9 AnalystJournal
The AnalystDOI
10.1039/c3an01674aPubMed ID
24106738Type
ArticleLanguage
enISSN
1364-5528ae974a485f413a2113503eed53cd6c53
10.1039/c3an01674a