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    Prevalence and heterogeneity of circulating tumour cells in metastatic cutaneous melanoma.

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    Authors
    Khoja, Leila
    Shenjere, Patrick
    Hodgson, Clare
    Hodgetts, Jackie
    Clack, G
    Hughes, A
    Lorigan, Paul C
    Dive, Caroline
    Affiliation
    Clinical and Experimental Pharmacology Group, Paterson Institute for Cancer Research.
    Issue Date
    2013-11-06
    
    Metadata
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    Abstract
    We previously demonstrated that circulating tumour cells (CTCs) are detectable by the MelCAM and high molecular weight melanoma-associated antigen (HMW-MAA)-dependent CellSearch platform. However, CTCs which do not express these capture and detection markers are not detectable by CellSearch. Consequently, we explored the use of isolation by size of epithelial tumour cells (ISET), a marker independent, filtration-based device to determine the prevalence and heterogeneity of CTCs in metastatic cutaneous melanoma patients. Ninety patients were prospectively recruited and blood samples taken before treatment. Patients' blood was filtered using the ISET platform. CTCs were enumerated using dual immunohistochemistry with positive selection by S100 expression and exclusion of leucocytes and endothelial cells expressing CD45 or CD144, respectively. A panel of markers (Melan-A, MITF, MelCAM, high molecular melanoma-associated antigen, CD271 and MAGEC) was also examined. Fifty-one patients (57%) had CTCs (range 1-44 CTCs/4 ml blood) and 12 patients also had circulating tumour microemboli. Seven patients had S100- CTCs, 11 patients' CTCs were S100+ and 33 patients had S100+ and S100- CTCs. Substantial intrapatient and interpatient heterogeneity was observed for all other melanoma-associated markers. CTCs in metastatic cutaneous melanoma are detectable using the flexible marker-independent ISET platform. CTCs display significant marker expression heterogeneity implying that marker-dependent platforms would not detect all CTCs and multimarker assays are now required to reveal the biological significance of this CTC heterogeneity.
    Citation
    Prevalence and heterogeneity of circulating tumour cells in metastatic cutaneous melanoma. 2013: Melanoma Res
    Journal
    Melanoma Research
    URI
    http://hdl.handle.net/10541/308802
    DOI
    10.1097/CMR.0000000000000025
    PubMed ID
    24201293
    Type
    Article
    Language
    en
    ISSN
    1473-5636
    ae974a485f413a2113503eed53cd6c53
    10.1097/CMR.0000000000000025
    Scopus Count
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