Chemically facilitating the generation of diagnostic ions from SUMO(2/3) remnant isopeptides.
AffiliationPaterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK; School of Chemistry, University of Manchester, Brunswick Street, Manchester, M13 9SU, UK.
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AbstractMapping sites of wild-type SUMO modification is a challenging endeavour. Here we postulate that a combination of chemical derivatistation and collision-induced dissociation (CID) could be used to generate SUMO remnant diagnostic ions to aid both detection of these isopeptides and increase the analytical value of the product ion spectra required to characterize the nature and position of modification.
CitationChemically facilitating the generation of diagnostic ions from SUMO(2/3) remnant isopeptides. 2013, 27 (18):2108-14 Rapid Commun Mass Spectrom
JournalRapid Communications in Mass Spectrometry
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