Role of phosphatidylinositol 5-phosphate 4-kinase α in zebrafish development.
dc.contributor.author | Elouarrat, D | |
dc.contributor.author | van der Velden, Y | |
dc.contributor.author | Jones, David R | |
dc.contributor.author | Moolenaar, W | |
dc.contributor.author | Divecha, Nullin | |
dc.contributor.author | Haramis, A | |
dc.date.accessioned | 2013-08-30T12:58:33Z | |
dc.date.available | 2013-08-30T12:58:33Z | |
dc.date.issued | 2013-07 | |
dc.identifier.citation | Role of phosphatidylinositol 5-phosphate 4-kinase α in zebrafish development. 2013, 45 (7):1293-301 Int J Biochem Cell Biol | en_GB |
dc.identifier.issn | 1878-5875 | |
dc.identifier.pmid | 23542014 | |
dc.identifier.doi | 10.1016/j.biocel.2013.03.009 | |
dc.identifier.uri | http://hdl.handle.net/10541/300400 | |
dc.description.abstract | Phosphatidylinositol 5-phosphate 4-kinases (PIP4Ks) phosphorylate phosphatidylinositol 5-phosphate (PI5P) to generate phosphatidylinositol 4,5-bisphosphate; their most likely function is the regulation of the levels of PI5P, a putative signalling intermediate. There are three mammalian PIP4Ks isoforms (α, β and γ), but their physiological roles remain poorly understood. In the present study, we identified the zebrafish orthologue (zPIP4Kα) of the high-activity human PIP4K α isoform and analyzed its role in embryonic development. RT-PCR analysis and whole-mount in situ hybridization experiments showed that zPIP4Kα is maternally expressed. At later embryonic stages, high PIP4Kα expression was detected in the head and the pectoral fins. Knockdown of zPIP4Kα by antisense morpholino oligonucleotides led to severe morphological abnormalities, including midbody winding defects at 48hpf. The abnormal phenotype could be rescued, at least in large part, by injection of human PIP4Kα mRNA. Our results reveal a key role for PIP4Kα and its activity in vertebrate tissue homeostasis and organ development. | |
dc.language.iso | en | en |
dc.rights | Archived with thanks to The international journal of biochemistry & cell biology | en_GB |
dc.title | Role of phosphatidylinositol 5-phosphate 4-kinase α in zebrafish development. | en |
dc.type | Article | en |
dc.contributor.department | Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. | en_GB |
dc.identifier.journal | International Journal of Biochemistry & Cell Biology | en_GB |
html.description.abstract | Phosphatidylinositol 5-phosphate 4-kinases (PIP4Ks) phosphorylate phosphatidylinositol 5-phosphate (PI5P) to generate phosphatidylinositol 4,5-bisphosphate; their most likely function is the regulation of the levels of PI5P, a putative signalling intermediate. There are three mammalian PIP4Ks isoforms (α, β and γ), but their physiological roles remain poorly understood. In the present study, we identified the zebrafish orthologue (zPIP4Kα) of the high-activity human PIP4K α isoform and analyzed its role in embryonic development. RT-PCR analysis and whole-mount in situ hybridization experiments showed that zPIP4Kα is maternally expressed. At later embryonic stages, high PIP4Kα expression was detected in the head and the pectoral fins. Knockdown of zPIP4Kα by antisense morpholino oligonucleotides led to severe morphological abnormalities, including midbody winding defects at 48hpf. The abnormal phenotype could be rescued, at least in large part, by injection of human PIP4Kα mRNA. Our results reveal a key role for PIP4Kα and its activity in vertebrate tissue homeostasis and organ development. |