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dc.contributor.authorElouarrat, Den_GB
dc.contributor.authorvan der Velden, Yen_GB
dc.contributor.authorJones, David Ren_GB
dc.contributor.authorMoolenaar, Wen_GB
dc.contributor.authorDivecha, Nullinen_GB
dc.contributor.authorHaramis, Aen_GB
dc.date.accessioned2013-08-30T12:58:33Z
dc.date.available2013-08-30T12:58:33Z
dc.date.issued2013-07
dc.identifier.citationRole of phosphatidylinositol 5-phosphate 4-kinase α in zebrafish development. 2013, 45 (7):1293-301 Int J Biochem Cell Biolen_GB
dc.identifier.issn1878-5875
dc.identifier.pmid23542014
dc.identifier.doi10.1016/j.biocel.2013.03.009
dc.identifier.urihttp://hdl.handle.net/10541/300400
dc.description.abstractPhosphatidylinositol 5-phosphate 4-kinases (PIP4Ks) phosphorylate phosphatidylinositol 5-phosphate (PI5P) to generate phosphatidylinositol 4,5-bisphosphate; their most likely function is the regulation of the levels of PI5P, a putative signalling intermediate. There are three mammalian PIP4Ks isoforms (α, β and γ), but their physiological roles remain poorly understood. In the present study, we identified the zebrafish orthologue (zPIP4Kα) of the high-activity human PIP4K α isoform and analyzed its role in embryonic development. RT-PCR analysis and whole-mount in situ hybridization experiments showed that zPIP4Kα is maternally expressed. At later embryonic stages, high PIP4Kα expression was detected in the head and the pectoral fins. Knockdown of zPIP4Kα by antisense morpholino oligonucleotides led to severe morphological abnormalities, including midbody winding defects at 48hpf. The abnormal phenotype could be rescued, at least in large part, by injection of human PIP4Kα mRNA. Our results reveal a key role for PIP4Kα and its activity in vertebrate tissue homeostasis and organ development.
dc.language.isoenen
dc.rightsArchived with thanks to The international journal of biochemistry & cell biologyen_GB
dc.titleRole of phosphatidylinositol 5-phosphate 4-kinase α in zebrafish development.en
dc.typeArticleen
dc.contributor.departmentDivision of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.en_GB
dc.identifier.journalInternational Journal of Biochemistry & Cell Biologyen_GB
html.description.abstractPhosphatidylinositol 5-phosphate 4-kinases (PIP4Ks) phosphorylate phosphatidylinositol 5-phosphate (PI5P) to generate phosphatidylinositol 4,5-bisphosphate; their most likely function is the regulation of the levels of PI5P, a putative signalling intermediate. There are three mammalian PIP4Ks isoforms (α, β and γ), but their physiological roles remain poorly understood. In the present study, we identified the zebrafish orthologue (zPIP4Kα) of the high-activity human PIP4K α isoform and analyzed its role in embryonic development. RT-PCR analysis and whole-mount in situ hybridization experiments showed that zPIP4Kα is maternally expressed. At later embryonic stages, high PIP4Kα expression was detected in the head and the pectoral fins. Knockdown of zPIP4Kα by antisense morpholino oligonucleotides led to severe morphological abnormalities, including midbody winding defects at 48hpf. The abnormal phenotype could be rescued, at least in large part, by injection of human PIP4Kα mRNA. Our results reveal a key role for PIP4Kα and its activity in vertebrate tissue homeostasis and organ development.


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