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    Role of phosphatidylinositol 5-phosphate 4-kinase α in zebrafish development.

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    Authors
    Elouarrat, D
    van der Velden, Y
    Jones, David R
    Moolenaar, W
    Divecha, Nullin
    Haramis, A
    Affiliation
    Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
    Issue Date
    2013-07
    
    Metadata
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    Abstract
    Phosphatidylinositol 5-phosphate 4-kinases (PIP4Ks) phosphorylate phosphatidylinositol 5-phosphate (PI5P) to generate phosphatidylinositol 4,5-bisphosphate; their most likely function is the regulation of the levels of PI5P, a putative signalling intermediate. There are three mammalian PIP4Ks isoforms (α, β and γ), but their physiological roles remain poorly understood. In the present study, we identified the zebrafish orthologue (zPIP4Kα) of the high-activity human PIP4K α isoform and analyzed its role in embryonic development. RT-PCR analysis and whole-mount in situ hybridization experiments showed that zPIP4Kα is maternally expressed. At later embryonic stages, high PIP4Kα expression was detected in the head and the pectoral fins. Knockdown of zPIP4Kα by antisense morpholino oligonucleotides led to severe morphological abnormalities, including midbody winding defects at 48hpf. The abnormal phenotype could be rescued, at least in large part, by injection of human PIP4Kα mRNA. Our results reveal a key role for PIP4Kα and its activity in vertebrate tissue homeostasis and organ development.
    Citation
    Role of phosphatidylinositol 5-phosphate 4-kinase α in zebrafish development. 2013, 45 (7):1293-301 Int J Biochem Cell Biol
    Journal
    International Journal of Biochemistry & Cell Biology
    URI
    http://hdl.handle.net/10541/300400
    DOI
    10.1016/j.biocel.2013.03.009
    PubMed ID
    23542014
    Type
    Article
    Language
    en
    ISSN
    1878-5875
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.biocel.2013.03.009
    Scopus Count
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    All Paterson Institute for Cancer Research

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