Exploring the spectroscopic differences of Caki-2 cells progressing through the cell cycle while proliferating in vitro.
Affiliation
Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK. peter.gardner@manchester.ac.uk.Issue Date
2013-07-21
Metadata
Show full item recordAbstract
FTIR micro-spectral images of Caki-2 cells cytospun onto calcium fluoride (CaF2) slides were used to build a computational model in order to discriminate between the biochemical events of the continuous cell cycle during proliferation. Multivariate analysis and machine learning techniques such as PCA, PLSR and SVMs were used to highlight the chemical differences among the cell cycle phases and also to point out the need for removing the distortion of the spectra due to the morphology of the cells. Results showed cell cycle dependant scattering profiles that enabled the training of a SVM in order to recognise, with a relative high accuracy, each cell cycle phase purely with the scattering curve removed from the FTIR data after being subject to the RMieS-EMSC algorithm.Citation
Exploring the spectroscopic differences of Caki-2 cells progressing through the cell cycle while proliferating in vitro. 2013, 138 (14):3957-66 AnalystJournal
The AnalystDOI
10.1039/c3an00507kPubMed ID
23640135Type
ArticleLanguage
enISSN
1364-5528ae974a485f413a2113503eed53cd6c53
10.1039/c3an00507k