A fluorescence-based assay for the apurinic/apyrimidinic-site cleavage activity of human tyrosyl-DNA phosphodiesterase 1.
Authors
Thomson, Graeme JHamilton, Nicola S
Hopkins, Gemma V
Waddell, Ian D
Watson, Amanda J
Ogilvie, Donald J
Affiliation
Cancer Research UK Drug Discovery Unit, Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, UK. Electronic address: gthomson@picr.man.ac.uk.Issue Date
2013-05-18
Metadata
Show full item recordAbstract
Tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the hydrolysis of phosphodiester bonds between the DNA 3'-phosphate and tyrosine residues and plays a major role in the repair of stalled topoisomerase I-DNA covalent complexes. Given this role, Tdp1 is of interest as a potential target for anticancer therapy. Inhibiting Tdp1 in combination with clinically used Top1 inhibitors may potentiate the effects of the latter and help to overcome some of the chemoresistance issues currently observed. In addition, Tdp1 can function during DNA repair to remove a variety of other 3' adducts from DNA such as phosphoglycolates and abasic or apurinic/apyrimidinic (AP) sites. Here we describe a new mix-and-read homogeneous fluorogenic assay for the measurement of the AP-site cleavage activity of Tdp1 that is compatible with high-throughput screening. The application of such an assay will open up further avenues for the discovery of novel Tdp1 inhibitors.Citation
A fluorescence-based assay for the apurinic/apyrimidinic-site cleavage activity of human tyrosyl-DNA phosphodiesterase 1. 2013, 440 (1):1-5 Anal BiochemJournal
Analytical BiochemistryDOI
10.1016/j.ab.2013.05.003PubMed ID
23688965Type
ArticleLanguage
enISSN
1096-0309ae974a485f413a2113503eed53cd6c53
10.1016/j.ab.2013.05.003