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dc.contributor.authorGupta, Aen_GB
dc.contributor.authorToscano, Sen_GB
dc.contributor.authorTrivedi, Den_GB
dc.contributor.authorJones, David Ren_GB
dc.contributor.authorMathre, Sen_GB
dc.contributor.authorClarke, Jen_GB
dc.contributor.authorDivecha, Nullinen_GB
dc.contributor.authorRaghu, Pen_GB
dc.date.accessioned2013-06-28T15:50:02Z
dc.date.available2013-06-28T15:50:02Z
dc.date.issued2013-04-09
dc.identifier.citationPhosphatidylinositol 5-phosphate 4-kinase (PIP4K) regulates TOR signaling and cell growth during drosophila development. 2013, 110 (15):5963-8 Proc Natl Acad Sci USAen_GB
dc.identifier.issn1091-6490
dc.identifier.pmid23530222
dc.identifier.doi10.1073/pnas.1219333110
dc.identifier.urihttp://hdl.handle.net/10541/294915
dc.description.abstractDuring development, Drosophila larvae undergo a dramatic increase in body mass wherein nutritional and developmental cues are transduced into growth through the activity of complex signaling pathways. Class I phosphoinositide 3-kinases have an established role in this process. In this study we identify Drosophila phosphatidylinositol 5-phosphate 4-kinase (dPIP4K) as a phosphoinositide kinase that regulates growth during larval development. Loss-of-function mutants in dPIP4K show reduced body weight and prolonged larval development, whereas overexpression of dPIP4K results both in an increase in body weight and shortening of larval development. The growth defect associated with dPIP4K loss of function is accompanied by a reduction in the average cell size of larval endoreplicative tissues. Our findings reveal that these phenotypes are underpinned by changes in the signaling input into the target of rapamycin (TOR) signaling complex and changes in the activity of its direct downstream target p70 S6 kinase. Together, these results define dPIP4K activity as a regulator of cell growth and TOR signaling during larval development.
dc.language.isoenen
dc.rightsArchived with thanks to Proceedings of the National Academy of Sciences of the United States of Americaen_GB
dc.titlePhosphatidylinositol 5-phosphate 4-kinase (PIP4K) regulates TOR signaling and cell growth during drosophila development.en
dc.typeArticleen
dc.contributor.departmentNational Centre for Biological Sciences, Tata Institute of Fundamental Research, Gandhi Krishi Vignana Kendra Campus, Bangalore 560065, India.en_GB
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen_GB
html.description.abstractDuring development, Drosophila larvae undergo a dramatic increase in body mass wherein nutritional and developmental cues are transduced into growth through the activity of complex signaling pathways. Class I phosphoinositide 3-kinases have an established role in this process. In this study we identify Drosophila phosphatidylinositol 5-phosphate 4-kinase (dPIP4K) as a phosphoinositide kinase that regulates growth during larval development. Loss-of-function mutants in dPIP4K show reduced body weight and prolonged larval development, whereas overexpression of dPIP4K results both in an increase in body weight and shortening of larval development. The growth defect associated with dPIP4K loss of function is accompanied by a reduction in the average cell size of larval endoreplicative tissues. Our findings reveal that these phenotypes are underpinned by changes in the signaling input into the target of rapamycin (TOR) signaling complex and changes in the activity of its direct downstream target p70 S6 kinase. Together, these results define dPIP4K activity as a regulator of cell growth and TOR signaling during larval development.


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