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dc.contributor.authorJervis, S
dc.contributor.authorCollins, P
dc.contributor.authorTate, D
dc.contributor.authorFoster, L
dc.contributor.authorBowman, V
dc.contributor.authorAdhern, C
dc.contributor.authorBloor, Adrian
dc.contributor.authorYin, J
dc.contributor.authorWynn, R
dc.contributor.authorPoulton, K
dc.date.accessioned2013-05-20T12:55:07Z
dc.date.available2013-05-20T12:55:07Z
dc.date.issued2013-04
dc.identifier.citationIncreased severity of acute graft versus host disease as a result of differential expression following a homozygous gene deletion. 2013, 40 (2):116-9 Int J Immunogeneten_GB
dc.identifier.issn1744-313X
dc.identifier.pmid22726315
dc.identifier.doi10.1111/j.1744-313X.2012.01138.x
dc.identifier.urihttp://hdl.handle.net/10541/292399
dc.description.abstractAcute graft versus host disease (aGvHD) is a major cause of early morbidity post-haematopoietic stem cell transplantation with minor histocompatibility antigens being a contributing factor. One mHA encoded by the UDP glycosyltransferase 2 family polypeptide B17 (UGT2B17) gene has been shown to be immunogenic because of differential expression in the donor and recipient. We investigated the effects of a homozygous gene deletion of UGT2B17 on the severity of acute aGvHD post-HSCT in HLA-matched related donors. 115 donor and recipient HLA and UGT2B17 genotypes were determined using PCR-SSO and PCR-SSP, respectively. aGvHD grading was determined using routine criteria and dichotomized into either nonclinically significant (0-I) or clinically significant (II-IV). For all analyses, P-values of ≤ 0.05 were considered significant. The frequency of the gene deletion within the total cohort tested was 29.1%. A significant increase in aGvHD severity (grades II-IV) was seen in UGT2B17 recipients expressing the protein when transplanted with a UGT2B17 disparate donor (P = 0.011). We observed a significant association between UGT2B17 expressing recipients and UGT2B17 deficient donors with the severity of aGvHD. This study provides additional evidence that genomic variations may predispose to more severe aGvHD, but are not a mechanism for GvHD.
dc.language.isoenen
dc.rightsArchived with thanks to International journal of immunogeneticsen_GB
dc.titleIncreased severity of acute graft versus host disease as a result of differential expression following a homozygous gene deletion.en
dc.typeArticleen
dc.contributor.departmentTransplantation Laboratory, Manchester Royal Infirmary, Manchester, UK. steven.jervis@cmft.nhs.uken_GB
dc.identifier.journalInternational Journal of Immunogeneticsen_GB
html.description.abstractAcute graft versus host disease (aGvHD) is a major cause of early morbidity post-haematopoietic stem cell transplantation with minor histocompatibility antigens being a contributing factor. One mHA encoded by the UDP glycosyltransferase 2 family polypeptide B17 (UGT2B17) gene has been shown to be immunogenic because of differential expression in the donor and recipient. We investigated the effects of a homozygous gene deletion of UGT2B17 on the severity of acute aGvHD post-HSCT in HLA-matched related donors. 115 donor and recipient HLA and UGT2B17 genotypes were determined using PCR-SSO and PCR-SSP, respectively. aGvHD grading was determined using routine criteria and dichotomized into either nonclinically significant (0-I) or clinically significant (II-IV). For all analyses, P-values of ≤ 0.05 were considered significant. The frequency of the gene deletion within the total cohort tested was 29.1%. A significant increase in aGvHD severity (grades II-IV) was seen in UGT2B17 recipients expressing the protein when transplanted with a UGT2B17 disparate donor (P = 0.011). We observed a significant association between UGT2B17 expressing recipients and UGT2B17 deficient donors with the severity of aGvHD. This study provides additional evidence that genomic variations may predispose to more severe aGvHD, but are not a mechanism for GvHD.


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