Identification of a functional prostanoid-like receptor in the protozoan parasite, trypanosoma cruzi.
Lisanti, Michael P
AffiliationDepartment of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, USA. firstname.lastname@example.org
MetadataShow full item record
AbstractTrypanosoma cruzi infection in humans and experimental animals causes Chagas disease which is often accompanied by myocarditis, cardiomyopathy, and vasculopathy. T. cruzi-derived thromboxane A2 (TXA2) modulates vasculopathy and other pathophysiological features of Chagasic cardiomyopathy. Here, we provide evidence that epimastigotes, trypomastigotes, and amastigotes of T. cruzi (Brazil and Tulahuen strains) express a biologically active prostanoid receptor (PR) that is responsive to TXA2 mimetics, e.g. IBOP. This putative receptor, TcPR, is mainly localized in the flagellar membrane of the parasites and shows a similar glycosylation pattern to that of bona fide thromboxane prostanoid (TP) receptors obtained from human platelets. Furthermore, TXA2-PR signal transduction activates T. cruzi-specific MAPK pathways. While mammalian TP is a G-protein coupled receptor (GPCR); T. cruzi genome sequencing has not demonstrated any confirmed GPCRs in these parasites. Based on this genome sequencing it is likely that TcPR is unique in these protists with no counterpart in mammals. TXA2 is a potent vasoconstrictor which contributes to the pathogenesis of Chagasic cardiovascular disease. It may, however, also control parasite differentiation and proliferation in the infected host allowing the infection to progress to a chronic state.
CitationIdentification of a functional prostanoid-like receptor in the protozoan parasite, trypanosoma cruzi. 2013, 112 (4):1417-25 Parasitol Res
- Microarray analysis of the mammalian thromboxane receptor-Trypanosoma cruzi interaction.
- Authors: Tanowitz HB, Mukhopadhyay A, Ashton AW, Lisanti MP, Machado FS, Weiss LM, Mukherjee S
- Issue date: 2011 Apr 1
- Trypanosoma cruzi Produces the Specialized Proresolving Mediators Resolvin D1, Resolvin D5, and Resolvin E2.
- Authors: Colas RA, Ashton AW, Mukherjee S, Dalli J, Akide-Ndunge OB, Huang H, Desruisseaux MS, Guan F, Jelicks LA, Matos Dos Santos F, Nagajyothi J, Zingman MA, Reyes J, Weiss LM, Serhan CN, Tanowitz HB
- Issue date: 2018 Apr
- A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
- Authors: Lu B, PereiraPerrin M
- Issue date: 2008 Aug
- Thromboxane A2 is a key regulator of pathogenesis during Trypanosoma cruzi infection.
- Authors: Ashton AW, Mukherjee S, Nagajyothi FN, Huang H, Braunstein VL, Desruisseaux MS, Factor SM, Lopez L, Berman JW, Wittner M, Scherer PE, Capra V, Coffman TM, Serhan CN, Gotlinger K, Wu KK, Weiss LM, Tanowitz HB
- Issue date: 2007 Apr 16
- Trypanosoma cruzi evades the protective role of interferon-gamma-signaling in parasite-infected cells.
- Authors: Stahl P, Ruppert V, Schwarz RT, Meyer T
- Issue date: 2014