Hof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis in budding yeast.
AffiliationPaterson Institute for Cancer Research, University of Manchester, Manchester, United Kingdom.
MetadataShow full item record
AbstractThe Hof1 protein (Homologue of Fifteen) regulates formation of the primary septum during cytokinesis in the budding yeast , whereas the orthologous Cdc15 protein in fission yeast regulates the actomyosin ring by using its F-BAR domain to recruit actin nucleators to the cleavage site. Here we show that budding yeast Hof1 also contributes to actin ring assembly in parallel with the Rvs167 protein. Simultaneous deletion of the and genes is lethal, and cells fail to assemble the actomyosin ring as they progress through mitosis. Although Hof1 and Rvs167 are not orthologues, they both share an analogous structure, with an F-BAR or BAR domain at the amino terminus, capable of inducing membrane curvature, and SH3 domains at the carboxyl terminus that bind to specific proline-rich targets. The SH3 domain of Rvs167 becomes essential for assembly of the actomyosin ring in cells lacking Hof1, suggesting that it helps to recruit a regulator of the actin cytoskeleton. This new function of Rvs167 appears to be independent of its known role as a regulator of the Arp2/3 actin nucleator, as actin ring assembly is not abolished by the simultaneous inactivation of Hof1 and Arp2/3. Instead we find that recruitment to the bud-neck of the Iqg1 actin regulator is defective in cells lacking Hof1 and Rvs167, though future studies will be needed to determine if this reflects a direct interaction between these factors. The redundant role of Hof1 in actin ring assembly suggests that the mechanism of actin ring assembly has been conserved to a greater extent across evolution than anticipated previously.
CitationHof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis in budding yeast. 2013, 8 (2):e57846 PLoS ONE
- Targeting and functional mechanisms of the cytokinesis-related F-BAR protein Hof1 during the cell cycle.
- Authors: Oh Y, Schreiter J, Nishihama R, Wloka C, Bi E
- Issue date: 2013 May
- Verprolin cytokinesis function mediated by the Hof one trap domain.
- Authors: Ren G, Wang J, Brinkworth R, Winsor B, Kobe B, Munn AL
- Issue date: 2005 Jul
- Role of the Hof1-Cyk3 interaction in cleavage-furrow ingression and primary-septum formation during yeast cytokinesis.
- Authors: Wang M, Nishihama R, Onishi M, Pringle JR
- Issue date: 2018 Mar 1
- Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck.
- Authors: Jendretzki A, Ciklic I, Rodicio R, Schmitz HP, Heinisch JJ
- Issue date: 2009 Oct
- Cdk1-dependent phosphorylation of Iqg1 governs actomyosin ring assembly prior to cytokinesis.
- Authors: Naylor SG, Morgan DO
- Issue date: 2014 Mar 1