A novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine.
Griffiths, John R
Smith, Duncan L
AffiliationPaterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK; School of Chemistry, University of Manchester, Brunswick Street, Manchester, M13 9SU, UK.
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AbstractIdentification of sites of protein SUMOylation is of great importance due its functional diversity within the cell. To date, most approaches to this problem rely on site-directed mutagenesis and/or highly specialised mass spectrometry approaches. We present a novel alternative approach to the site mapping of SUMOylation using trypsin and elastase digestion, routine mass spectrometry and an unbiased isotag database searching strategy.
CitationA novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine. 2013, 27 (1):127-34 Rapid Commun Mass Spectrom
JournalRapid Communications in Mass Spectrometry
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