Authors
Evans, CNoirel, J
Ow, S
Salim, M
Pereira-Medrano, A
Couto, N
Pandhal, J
Smith, Duncan L
Pham, T
Karunakaran, E
Zou, X
Biggs, C
Wright, P
Affiliation
The ChELSI Institute, Chemical and Biological Engineering, The University of Sheffield, Sheffield, UK.Issue Date
2012-09
Metadata
Show full item recordAbstract
The iTRAQ (isobaric tags for relative and absolute quantification) technique is widely employed in proteomic workflows requiring relative quantification. Here, we review the iTRAQ literature; in particular, we focus on iTRAQ usage in relation to other commonly used quantitative techniques e.g. stable isotope labelling in culture (SILAC), label-free methods and selected reaction monitoring (SRM). As a result, we identify several issues arising with respect to iTRAQ. Perhaps frustratingly, iTRAQ's attractiveness has been undermined by a number of technical and analytical limitations: it may not be truly quantitative, as the changes in abundance reported will generally be underestimated. We discuss weaknesses and strengths of iTRAQ as a methodology for relative quantification in the light of this and other technical issues. We focus on technical developments targeted at iTRAQ accuracy and precision, use of 4-plex over 8-plex reagents and application of iTRAQ to post-translational modification (PTM) workflows. We also discuss iTRAQ in relation to label-free approaches, to which iTRAQ is losing ground.Citation
An insight into iTRAQ: where do we stand now? 2012, 404 (4):1011-27 Anal Bioanal ChemJournal
Analytical and Bioanalytical ChemistryDOI
10.1007/s00216-012-5918-6PubMed ID
22451173Type
ArticleLanguage
enISSN
1618-2650ae974a485f413a2113503eed53cd6c53
10.1007/s00216-012-5918-6
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