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    The Mitotic Exit Network and Cdc14 phosphatase initiate cytokinesis by counteracting CDK phosphorylations and blocking polarised growth.

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    Authors
    Sanchez-Diaz, Alberto
    Nkosi, Pedro Junior
    Murray, Stephen M
    Labib, Karim
    Affiliation
    Paterson Institute for Cancer Research, University of Manchester, Manchester, UK.
    Issue Date
    2012-08-29
    
    Metadata
    Show full item record
    Abstract
    Polarisation of the actin cytoskeleton must cease during cytokinesis, to support efficient assembly and contraction of the actomyosin ring at the site of cell division, but the underlying mechanisms are still understood poorly in most species. In budding yeast, the Mitotic Exit Network (MEN) releases Cdc14 phosphatase from the nucleolus during anaphase, leading to the inactivation of mitotic forms of cyclin-dependent kinase (CDK) and the onset of septation, before G1-CDK can be reactivated and drive re-polarisation of the actin cytoskeleton to a new bud. Here, we show that premature inactivation of mitotic CDK, before release of Cdc14, allows G1-CDK to divert the actin cytoskeleton away from the actomyosin ring to a new site of polarised growth, thereby delaying progression through cytokinesis. Our data indicate that cells normally avoid this problem via the MEN-dependent release of Cdc14, which counteracts all classes of CDK-mediated phosphorylations during cytokinesis and blocks polarised growth. The dephosphorylation of CDK targets is therefore central to the mechanism by which the MEN and Cdc14 initiate cytokinesis and block polarised growth during late mitosis.
    Citation
    The Mitotic Exit Network and Cdc14 phosphatase initiate cytokinesis by counteracting CDK phosphorylations and blocking polarised growth. 2012, 31 (17):3620-34 EMBO J
    Journal
    EMBO Journal
    URI
    http://hdl.handle.net/10541/254584
    DOI
    10.1038/emboj.2012.224
    PubMed ID
    22872148
    Type
    Article
    Language
    en
    ISSN
    1460-2075
    ae974a485f413a2113503eed53cd6c53
    10.1038/emboj.2012.224
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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