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dc.contributor.authorBitton, Danny A
dc.contributor.authorGrallert, Agnes
dc.contributor.authorScutt, Paul J
dc.contributor.authorYates, Tim
dc.contributor.authorLi, Yaoyong
dc.contributor.authorBradford, James R
dc.contributor.authorHey, Yvonne
dc.contributor.authorPepper, Stuart D
dc.contributor.authorHagan, Iain M
dc.contributor.authorMiller, Crispin J
dc.date.accessioned2012-11-12T16:42:07Z
dc.date.available2012-11-12T16:42:07Z
dc.date.issued2011
dc.identifier.citationProgrammed fluctuations in sense/antisense transcript ratios drive sexual differentiation in S. pombe. 2011, 7:559 Mol Syst Biolen_GB
dc.identifier.issn1744-4292
dc.identifier.pmid22186733
dc.identifier.doi10.1038/msb.2011.90
dc.identifier.urihttp://hdl.handle.net/10541/251862
dc.description.abstractStrand-specific RNA sequencing of S. pombe revealed a highly structured programme of ncRNA expression at over 600 loci. Waves of antisense transcription accompanied sexual differentiation. A substantial proportion of ncRNA arose from mechanisms previously considered to be largely artefactual, including improper 3' termination and bidirectional transcription. Constitutive induction of the entire spk1+, spo4+, dis1+ and spo6+ antisense transcripts from an integrated, ectopic, locus disrupted their respective meiotic functions. This ability of antisense transcripts to disrupt gene function when expressed in trans suggests that cis production at native loci during sexual differentiation may also control gene function. Consistently, insertion of a marker gene adjacent to the dis1+ antisense start site mimicked ectopic antisense expression in reducing the levels of this microtubule regulator and abolishing the microtubule-dependent 'horsetail' stage of meiosis. Antisense production had no impact at any of these loci when the RNA interference (RNAi) machinery was removed. Thus, far from being simply 'genome chatter', this extensive ncRNA landscape constitutes a fundamental component in the controls that drive the complex programme of sexual differentiation in S. pombe.
dc.language.isoenen
dc.rightsArchived with thanks to Molecular systems biologyen_GB
dc.subject.meshDatabases, Nucleic Acid
dc.subject.meshGene Expression Regulation, Fungal
dc.subject.meshGenes, Fungal
dc.subject.meshMeiosis
dc.subject.meshMicrobiological Phenomena
dc.subject.meshRNA, Antisense
dc.subject.meshRNA, Fungal
dc.subject.meshRNA, Small Interfering
dc.subject.meshRNA, Untranslated
dc.subject.meshSchizosaccharomyces
dc.subject.meshSystems Biology
dc.subject.meshTranscription, Genetic
dc.titleProgrammed fluctuations in sense/antisense transcript ratios drive sexual differentiation in S. pombe.en
dc.typeArticleen
dc.contributor.departmentCRUK Applied Computational Biology and Bioinformatics Group, Cancer Research UK, Paterson Institute for Cancer Research, The University of Manchester, Manchester, UK.en_GB
dc.identifier.journalMolecular Systems Biologyen_GB
html.description.abstractStrand-specific RNA sequencing of S. pombe revealed a highly structured programme of ncRNA expression at over 600 loci. Waves of antisense transcription accompanied sexual differentiation. A substantial proportion of ncRNA arose from mechanisms previously considered to be largely artefactual, including improper 3' termination and bidirectional transcription. Constitutive induction of the entire spk1+, spo4+, dis1+ and spo6+ antisense transcripts from an integrated, ectopic, locus disrupted their respective meiotic functions. This ability of antisense transcripts to disrupt gene function when expressed in trans suggests that cis production at native loci during sexual differentiation may also control gene function. Consistently, insertion of a marker gene adjacent to the dis1+ antisense start site mimicked ectopic antisense expression in reducing the levels of this microtubule regulator and abolishing the microtubule-dependent 'horsetail' stage of meiosis. Antisense production had no impact at any of these loci when the RNA interference (RNAi) machinery was removed. Thus, far from being simply 'genome chatter', this extensive ncRNA landscape constitutes a fundamental component in the controls that drive the complex programme of sexual differentiation in S. pombe.


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