Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells.
Affiliation
Centre for Biochemistry, Drug Design and Cancer Research, School of Environment & Life Sciences, University of Salford, Salford, UK.Issue Date
2011-10
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We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.Citation
Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells. 2011, 74 (4):419-22 Scand. J. Immunol.Journal
Scandinavian Journal of ImmunologyDOI
10.1111/j.1365-3083.2011.02588.xPubMed ID
21645031Type
ArticleLanguage
enISSN
1365-3083ae974a485f413a2113503eed53cd6c53
10.1111/j.1365-3083.2011.02588.x
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