Show simple item record

dc.contributor.authorYoshihara, S
dc.contributor.authorTakahashi, H
dc.contributor.authorNishimura, N
dc.contributor.authorNaritsuka, H
dc.contributor.authorShirao, T
dc.contributor.authorHirai, H
dc.contributor.authorYoshihara, Y
dc.contributor.authorMori, K
dc.contributor.authorStern, Peter L
dc.contributor.authorTsuboi, A
dc.date.accessioned2012-05-30T12:43:41Z
dc.date.available2012-05-30T12:43:41Z
dc.date.issued2012-02-08
dc.identifier.citation5T4 glycoprotein regulates the sensory input-dependent development of a specific subtype of newborn interneurons in the mouse olfactory bulb. 2012, 32 (6):2217-26 J Neuroscien_GB
dc.identifier.issn1529-2401
dc.identifier.pmid22323733
dc.identifier.doi10.1523/JNEUROSCI.5907-11.2012
dc.identifier.urihttp://hdl.handle.net/10541/226754
dc.description.abstractSensory input has been shown to regulate development in a variety of species and in various structures, including the retina, cortex, and olfactory bulb (OB). Within the mammalian OB specifically, the development of dendrites in mitral/tufted cells is well known to be odor-evoked activity dependent. However, little is known about the developmental role of sensory input in the other major OB population of the GABAgenic interneurons, such as granule cells and periglomerular cells. Here, we identified, with DNA microarray and in situ hybridization screenings, a trophoblast glycoprotein gene, 5T4, whose expression in a specific subtype of OB interneurons is dependent on sensory input. 5T4 is a type I membrane protein, whose extracellular domain contains seven leucine-rich repeats (LRR) flanked by characteristic LRR-N-flanking and C-flanking regions, and a cytoplasmic domain. 5T4 overexpression in the newborn OB interneurons facilitated their dendritic arborization even under the sensory input-deprived condition. By contrast, both 5T4 knockdown with RNAi and 5T4 knockout with mice resulted in a significant reduction in the dendritic arborization of 5T4(+) granule cells. Further, we identified the amino acid sequence in the 5T4 cytoplasmic domain that is necessary and sufficient for the sensory input-dependent dendritic shaping of specific neuronal subtypes in the OB. Thus, these results demonstrate that 5T4 glycoprotein contributes in the regulation of activity-dependent dendritic development of interneurons and the formation of functional neural circuitry in the OB.
dc.language.isoenen
dc.rightsArchived with thanks to The Journal of neuroscience : the official journal of the Society for Neuroscienceen_GB
dc.subject.meshAnimals
dc.subject.meshAntigens, Surface
dc.subject.meshBase Sequence
dc.subject.meshCell Differentiation
dc.subject.meshDendrites
dc.subject.meshFemale
dc.subject.meshInterneurons
dc.subject.meshMale
dc.subject.meshMembrane Glycoproteins
dc.subject.meshMice
dc.subject.meshMice, Inbred C57BL
dc.subject.meshMice, Inbred ICR
dc.subject.meshMice, Knockout
dc.subject.meshMolecular Sequence Data
dc.subject.meshNerve Net
dc.subject.meshOlfactory Bulb
dc.subject.meshSensory Deprivation
dc.title5T4 glycoprotein regulates the sensory input-dependent development of a specific subtype of newborn interneurons in the mouse olfactory bulb.en
dc.typeArticleen
dc.contributor.departmentLaboratory for Molecular Biology of Neural System, Advanced Medical Research Center, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.en_GB
dc.identifier.journalThe Journal of Neuroscienceen_GB
html.description.abstractSensory input has been shown to regulate development in a variety of species and in various structures, including the retina, cortex, and olfactory bulb (OB). Within the mammalian OB specifically, the development of dendrites in mitral/tufted cells is well known to be odor-evoked activity dependent. However, little is known about the developmental role of sensory input in the other major OB population of the GABAgenic interneurons, such as granule cells and periglomerular cells. Here, we identified, with DNA microarray and in situ hybridization screenings, a trophoblast glycoprotein gene, 5T4, whose expression in a specific subtype of OB interneurons is dependent on sensory input. 5T4 is a type I membrane protein, whose extracellular domain contains seven leucine-rich repeats (LRR) flanked by characteristic LRR-N-flanking and C-flanking regions, and a cytoplasmic domain. 5T4 overexpression in the newborn OB interneurons facilitated their dendritic arborization even under the sensory input-deprived condition. By contrast, both 5T4 knockdown with RNAi and 5T4 knockout with mice resulted in a significant reduction in the dendritic arborization of 5T4(+) granule cells. Further, we identified the amino acid sequence in the 5T4 cytoplasmic domain that is necessary and sufficient for the sensory input-dependent dendritic shaping of specific neuronal subtypes in the OB. Thus, these results demonstrate that 5T4 glycoprotein contributes in the regulation of activity-dependent dendritic development of interneurons and the formation of functional neural circuitry in the OB.


This item appears in the following Collection(s)

Show simple item record