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dc.contributor.authorWilliamson, Andrew J K
dc.contributor.authorWhetton, Anthony D
dc.date.accessioned2012-01-09T23:37:07Z
dc.date.available2012-01-09T23:37:07Z
dc.date.issued2011-10
dc.identifier.citationThe requirement for proteomics to unravel stem cell regulatory mechanisms. 2011, 226 (10):2478-83 J. Cell. Physiol.en
dc.identifier.issn1097-4652
dc.identifier.pmid21792904
dc.identifier.doi10.1002/jcp.22610
dc.identifier.urihttp://hdl.handle.net/10541/201132
dc.description.abstractStem cells are defined by their ability to self-renew and to differentiate, the processes whereby these events are achieved is the subject of much investigation. These studies include cancer stem cell populations, where eradication of this specific population is the ultimate goal of treatment. Whilst cellular signalling events and transcription factor complex-mediated changes in gene expression have been analysed in some detail within stem cells, full systematic understanding of the events promoting self-renewal or the commitment process leading to formation of a specific cell type require a systems biology approach. This in turn demands a need for proteomic analysis of post-translational regulation of protein levels, protein interactions, protein post-translational modification (e.g. ubiquitination, methylation, acetylation, phosphorylation) to identify networks for stem cell regulation. Furthermore, the phenomenon of induced pluripotency via cellular reprogramming also can be understood optimally using combined molecular biology and proteomics approaches; here we describe current research employing proteomics and mass spectrometry to dissect stem cell regulatory mechanisms.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCell Differentiation
dc.subject.meshCell Division
dc.subject.meshChromatography, Liquid
dc.subject.meshHumans
dc.subject.meshPluripotent Stem Cells
dc.subject.meshProtein Processing, Post-Translational
dc.subject.meshProteomics
dc.subject.meshTandem Mass Spectrometry
dc.titleThe requirement for proteomics to unravel stem cell regulatory mechanisms.en
dc.typeArticleen
dc.contributor.departmentStem Cell and Leukaemia Proteomics Laboratory, School of Cancer and Enabling Sciences, Manchester Academic Health Science Centre, The University of Manchester, Christie's NHS Foundation Trust, Wolfson Molecular Imaging Centre, Withington, Manchester, UK.en
dc.identifier.journalJournal of Cellular Physiologyen
html.description.abstractStem cells are defined by their ability to self-renew and to differentiate, the processes whereby these events are achieved is the subject of much investigation. These studies include cancer stem cell populations, where eradication of this specific population is the ultimate goal of treatment. Whilst cellular signalling events and transcription factor complex-mediated changes in gene expression have been analysed in some detail within stem cells, full systematic understanding of the events promoting self-renewal or the commitment process leading to formation of a specific cell type require a systems biology approach. This in turn demands a need for proteomic analysis of post-translational regulation of protein levels, protein interactions, protein post-translational modification (e.g. ubiquitination, methylation, acetylation, phosphorylation) to identify networks for stem cell regulation. Furthermore, the phenomenon of induced pluripotency via cellular reprogramming also can be understood optimally using combined molecular biology and proteomics approaches; here we describe current research employing proteomics and mass spectrometry to dissect stem cell regulatory mechanisms.


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