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    PHA stimulation of separated human lymphocyte populations.

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    Authors
    Potter, M R
    Moore, Michael
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
    Issue Date
    1975-09
    
    Metadata
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    Abstract
    Lymphocyte preparations from peripheral blood and tonsils were separated into populations enriched with T or B cells by formation of rosettes with SRBC and separation of the rosette-forming and non-rosette-forming populations. T cell-enriched populations were also prepared by nylon column filtration. Using these methods preparations were obtained which comprised 80--95% T or B lymphocytes as determined by E-rosette formation and surface immunoglobulin (Ig) staining. PHA responsiveness, measured by [3H]thymidine incorporation, varied between relatively wide limits and was critically dependent on the degree of separation obtained. Relatively pure B-cell populations (less than 12% T cells) from blood and tonsils gave low PHA responses while preparations from blood still containing 24--38% T cells gave responses equal to or even greater than those of unseparated controls (60--78% T cells). T cell-enriched populations (80--86% T cells) responded to an equal or greater degree than controls but more efficient separation (greater than 90% T cells) resulted in markedly reduced stimulation. There was thus no simple correlation between the degree of phytomitogen-induced transformation and the number of T cells present. It is concluded that the low response of relatively pure T-cell populations may be due to depletion of B cells or non-lymphoid cells (or both) during the separation procedures. These observations have implications for the use of PHA stimulation as a measure of T-cell activity in mixed populations such as those of human peripheral blood leucocytes.
    Citation
    PHA stimulation of separated human lymphocyte populations. 1975, 21 (3):456-67 Clin. Exp. Immunol.
    Journal
    Clinical and Experimental Immunology
    URI
    http://hdl.handle.net/10541/199371
    PubMed ID
    1106926
    Type
    Article
    Language
    en
    ISSN
    0009-9104
    Collections
    All Paterson Institute for Cancer Research

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