• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    An electron microscope study of mitochondrial DNA in spontaneous human tumours and chemically induced animal tumours.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Kumar, Patricia
    Fox, Brian W
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
    Issue Date
    1974-06
    
    Metadata
    Show full item record
    Abstract
    MtDNA was extracted by a phenol method from transplanted and primary DAB induced hepatomata in male Wistar rats, normal rat liver, spontaneous human tumours (2 Wilm's tumours, one neuroblastoma and one adrenal carcinoma), as well as 2 specimens of normal human kidney, BNU induced "leukaemias" in mice and CHO fibroblasts in monolayer culture. The proportion of monomers, catenated dimers and oligomers, open dimers and small circles was determined by electron microscopy of the fractions comprising lower and middle DNA bands in a CsCl-EthBr gradient. Tumours were compared where possible with their normal tissue of origin. Open dimers were found in 2 Wilm's tumours and their attached "normal-looking" kidney tissue but not in normal, non-malignant kidney or any other tissue studied. In Wilm's tumours, the occurrence of open dimers is far from being an all-or-none phenomenon. Malignancy produced little change in the relative proportions of catenated dimers and oligomers in the tissues studied. Small circles were found associated with mtDNA from every tissue. Tumour mtDNA was not more heterogeneous in length than monomers from the corresponding normal tissue, neither was the mean length of tumour mtDNA significantly different from its corresponding normal mtDNA.
    Citation
    An electron microscope study of mitochondrial DNA in spontaneous human tumours and chemically induced animal tumours. 1974, 29 (6):447-61 Br. J. Cancer
    Journal
    British Journal of Cancer
    URI
    http://hdl.handle.net/10541/199362
    PubMed ID
    4368398
    PubMed Central ID
    PMC2009120
    Type
    Article
    Language
    en
    ISSN
    0007-0920
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Physicochemical characterization of Novikoff hepatoma mitochondrial DNA.
    • Authors: White MT, Wagner EK, Tewari KK
    • Issue date: 1975 Apr
    • Single strand-containing replicating molecules of circular mitochondrial DNA.
    • Authors: Wolstenholme DR, Koike K, Cochran-Fouts P
    • Issue date: 1973 Jan
    • Estimation of tissue DNA content in Wilm's tumour.
    • Authors: Sharma SP, Gopal SC, Gangopadhyay AN, Gupta DK, Chansuria JP
    • Issue date: 1994 Jul-Aug
    • Changes in mitochondrial DNA in cardiac hypertrophy in the rat.
    • Authors: Rajamanickam C, Merten S, Kwiatkowska-Patzer B, Chuang CH, Zak R, Rabinowitz M
    • Issue date: 1979 Oct
    • Buoyant densities of mitochondrial DNA from tumour and normal tissues.
    • Authors: Wunderlich V, Böttger M
    • Issue date: 1973 May
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.