An electron microscope study of mitochondrial DNA in spontaneous human tumours and chemically induced animal tumours.
Affiliation
Paterson Laboratories, Christie Hospital and Holt Radium Institute, ManchesterIssue Date
1974-06
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Show full item recordAbstract
MtDNA was extracted by a phenol method from transplanted and primary DAB induced hepatomata in male Wistar rats, normal rat liver, spontaneous human tumours (2 Wilm's tumours, one neuroblastoma and one adrenal carcinoma), as well as 2 specimens of normal human kidney, BNU induced "leukaemias" in mice and CHO fibroblasts in monolayer culture. The proportion of monomers, catenated dimers and oligomers, open dimers and small circles was determined by electron microscopy of the fractions comprising lower and middle DNA bands in a CsCl-EthBr gradient. Tumours were compared where possible with their normal tissue of origin. Open dimers were found in 2 Wilm's tumours and their attached "normal-looking" kidney tissue but not in normal, non-malignant kidney or any other tissue studied. In Wilm's tumours, the occurrence of open dimers is far from being an all-or-none phenomenon. Malignancy produced little change in the relative proportions of catenated dimers and oligomers in the tissues studied. Small circles were found associated with mtDNA from every tissue. Tumour mtDNA was not more heterogeneous in length than monomers from the corresponding normal tissue, neither was the mean length of tumour mtDNA significantly different from its corresponding normal mtDNA.Citation
An electron microscope study of mitochondrial DNA in spontaneous human tumours and chemically induced animal tumours. 1974, 29 (6):447-61 Br. J. CancerJournal
British Journal of CancerPubMed ID
4368398PubMed Central ID
PMC2009120Type
ArticleLanguage
enISSN
0007-0920Collections
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