The sensitivity of HeLa and Chinese hamster (ovary) cells to methylene dimethane sulphonate.
Abstract
HeLa and Chinese hamster (ovary) cells were exposed in vitro to methylene dimethanesulphonate (MDMS) and their survival of colony-forming ability was assayed in monolayer culture. Asynchronous cultures were exposed to the drug for the whole culture period but cell survival was complicated by the toxicity of formaldehyde which is a final breakdown product of the drug. A short treatment schedule of 15 min within the hydrolytic half life of the agent was therefore employed and the response of synchronous cultures of HeLa cells was then assayed throughout the course of the cells cycle. Cells were most sensitive at the beginning of the DNA synthetic phase (early-S) and most resistant at the end (late-S).Citation
The sensitivity of HeLa and Chinese hamster (ovary) cells to methylene dimethane sulphonate. 1976, 14 (1-2):93-9 Chem Biol InteractJournal
Chemico-Biological InteractionsDOI
10.1016/0009-2797(76)90027-2PubMed ID
182391Type
ArticleLanguage
enISSN
0009-2797ae974a485f413a2113503eed53cd6c53
10.1016/0009-2797(76)90027-2