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dc.contributor.authorNias, A H
dc.contributor.authorFox, Brian W
dc.date.accessioned2011-11-25T12:02:08Z
dc.date.available2011-11-25T12:02:08Z
dc.date.issued1976-07
dc.identifier.citationThe sensitivity of HeLa and Chinese hamster (ovary) cells to methylene dimethane sulphonate. 1976, 14 (1-2):93-9 Chem Biol Interacten
dc.identifier.issn0009-2797
dc.identifier.issn10.1016/0009-2797(76)90027-2
dc.identifier.pmid182391
dc.identifier.urihttp://hdl.handle.net/10541/190830
dc.description.abstractHeLa and Chinese hamster (ovary) cells were exposed in vitro to methylene dimethanesulphonate (MDMS) and their survival of colony-forming ability was assayed in monolayer culture. Asynchronous cultures were exposed to the drug for the whole culture period but cell survival was complicated by the toxicity of formaldehyde which is a final breakdown product of the drug. A short treatment schedule of 15 min within the hydrolytic half life of the agent was therefore employed and the response of synchronous cultures of HeLa cells was then assayed throughout the course of the cells cycle. Cells were most sensitive at the beginning of the DNA synthetic phase (early-S) and most resistant at the end (late-S).
dc.language.isoenen
dc.subject.meshCell Division
dc.subject.meshCell Line
dc.subject.meshCell Survival
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshHela Cells
dc.subject.meshMesylates
dc.subject.meshMethyl Methanesulfonate
dc.subject.meshMitosis
dc.subject.meshTime Factors
dc.titleThe sensitivity of HeLa and Chinese hamster (ovary) cells to methylene dimethane sulphonate.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX Great Britainen
dc.identifier.journalChemico-Biological Interactionsen
html.description.abstractHeLa and Chinese hamster (ovary) cells were exposed in vitro to methylene dimethanesulphonate (MDMS) and their survival of colony-forming ability was assayed in monolayer culture. Asynchronous cultures were exposed to the drug for the whole culture period but cell survival was complicated by the toxicity of formaldehyde which is a final breakdown product of the drug. A short treatment schedule of 15 min within the hydrolytic half life of the agent was therefore employed and the response of synchronous cultures of HeLa cells was then assayed throughout the course of the cells cycle. Cells were most sensitive at the beginning of the DNA synthetic phase (early-S) and most resistant at the end (late-S).


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