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dc.contributor.authorJeney, A
dc.contributor.authorFox, Brian W
dc.contributor.authorGarrett, J V
dc.contributor.authorFox, Margaret
dc.contributor.authorHolland, J
dc.date.accessioned2011-11-25T11:45:30Z
dc.date.available2011-11-25T11:45:30Z
dc.date.issued1976-12
dc.identifier.citationThe effect of dibromodulcitol on resting and dividing lymphoid cells. 1976, 15 (4):299-307 Chem Biol Interacten
dc.identifier.issn0009-2797
dc.identifier.pmid1009628
dc.identifier.doi1016/0009-2797(76)90135-6
dc.identifier.urihttp://hdl.handle.net/10541/190812
dc.description.abstractThe effect of dibromodulcitol (DBD) on the incorporation of labelled precursors into DNA and RNA fractions of PHA-stimulated human lymphocytes and of P388F lymphoma cells at various stages of their growth was studied. Both cell systems showed sensitivity to the drug within the concentration rage of 1-10 mug/ml. When DBD was added before phytohaemagglutinin (PHA), h.han RNA. In contrast, by adding DBD after PHA, RNA labelling was much more inhibited than DNA. In the latter case, the decrease in DNA labelling occurred only 24 h after drug treatment whereas RNA labelling was decreased 1 h after treatment. Levels of DBD which normally produced 30% inhibition in plating efficiency of P388F lymphoma cells affected uridine-5-T incorporation to a different extent at different stages of growth of the culture. Enhanced RNA labelling occurred in early exponential stage while at later stages of growth, RNA synthesis was depressed.
dc.language.isoenen
dc.subject.meshAdult
dc.subject.meshCell Division
dc.subject.meshCell Line
dc.subject.meshHumans
dc.subject.meshIdoxuridine
dc.subject.meshKinetics
dc.subject.meshLectins
dc.subject.meshLymphocyte Activation
dc.subject.meshLymphocytes
dc.subject.meshLymphoma
dc.subject.meshMale
dc.subject.meshMitolactol
dc.subject.meshTranscription, Genetic
dc.titleThe effect of dibromodulcitol on resting and dividing lymphoid cells.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX Great Britainen
dc.identifier.journalChemico-Biological Interactionsen
html.description.abstractThe effect of dibromodulcitol (DBD) on the incorporation of labelled precursors into DNA and RNA fractions of PHA-stimulated human lymphocytes and of P388F lymphoma cells at various stages of their growth was studied. Both cell systems showed sensitivity to the drug within the concentration rage of 1-10 mug/ml. When DBD was added before phytohaemagglutinin (PHA), h.han RNA. In contrast, by adding DBD after PHA, RNA labelling was much more inhibited than DNA. In the latter case, the decrease in DNA labelling occurred only 24 h after drug treatment whereas RNA labelling was decreased 1 h after treatment. Levels of DBD which normally produced 30% inhibition in plating efficiency of P388F lymphoma cells affected uridine-5-T incorporation to a different extent at different stages of growth of the culture. Enhanced RNA labelling occurred in early exponential stage while at later stages of growth, RNA synthesis was depressed.


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