The effect of dibromodulcitol on resting and dividing lymphoid cells.
Affiliation
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX Great BritainIssue Date
1976-12
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The effect of dibromodulcitol (DBD) on the incorporation of labelled precursors into DNA and RNA fractions of PHA-stimulated human lymphocytes and of P388F lymphoma cells at various stages of their growth was studied. Both cell systems showed sensitivity to the drug within the concentration rage of 1-10 mug/ml. When DBD was added before phytohaemagglutinin (PHA), h.han RNA. In contrast, by adding DBD after PHA, RNA labelling was much more inhibited than DNA. In the latter case, the decrease in DNA labelling occurred only 24 h after drug treatment whereas RNA labelling was decreased 1 h after treatment. Levels of DBD which normally produced 30% inhibition in plating efficiency of P388F lymphoma cells affected uridine-5-T incorporation to a different extent at different stages of growth of the culture. Enhanced RNA labelling occurred in early exponential stage while at later stages of growth, RNA synthesis was depressed.Citation
The effect of dibromodulcitol on resting and dividing lymphoid cells. 1976, 15 (4):299-307 Chem Biol InteractJournal
Chemico-Biological InteractionsDOI
1016/0009-2797(76)90135-6PubMed ID
1009628Type
ArticleLanguage
enISSN
0009-2797ae974a485f413a2113503eed53cd6c53
1016/0009-2797(76)90135-6
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