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dc.contributor.authorO'Connor, Peter J
dc.contributor.authorSaffhill, Roy
dc.date.accessioned2011-11-21T12:45:32Z
dc.date.available2011-11-21T12:45:32Z
dc.date.issued1979-06
dc.identifier.citationThe action of rat cytosol enzymes on some methylated nucleic acid components produced by the carcinogenic N-nitroso compounds. 1979, 26 (1):91-102 Chem Biol Interacten
dc.identifier.issn0009-2797
dc.identifier.pmid466746
dc.identifier.doi10.1016/0009-2797(79)90095-4
dc.identifier.urihttp://hdl.handle.net/10541/190112
dc.description.abstractThe stabilities of several alkylated nucleic acid components have been examined in the presence of cytosol extracts from a variety of rat tissues. An activity capable of demethylating O6-methyldeoxyguanosine was readily detectable in all tissues examined; arranged in approximate order of decreasing specific activity these are as follows: small intestine, spleen, kidney, lung, liver, skin, heart and brain. The in vitro requirements for the activity derived from liver and the observations that O6-methylguanine and its deoxynucleoside 5'-monophosphate are insensitive to the action of these extracts suggests that this activity may be due to an enzyme which resembles adenosine deaminase. In contrast to the ready degradation of O6-methyldeoxyguanosine the corresponding ethyl derivative was degraded very much more slowly but there was no evidence for other activities against the O4- and O2-methyldeoxythymidines. Similarly, no demethylation of the N-substituted deoxynucleosides, 3-methyldeoxycytidine 3-methldeoxythymidine, 1-methyldeoxyadenosine and 7-methyldeoxyguanosine, was detected.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCarcinogens
dc.subject.meshCytosol
dc.subject.meshDeoxyguanosine
dc.subject.meshDeoxyribonucleosides
dc.subject.meshHydrolysis
dc.subject.meshLiver
dc.subject.meshMale
dc.subject.meshMethylation
dc.subject.meshNitroso Compounds
dc.subject.meshRats
dc.subject.meshTissue Distribution
dc.titleThe action of rat cytosol enzymes on some methylated nucleic acid components produced by the carcinogenic N-nitroso compounds.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchesteren
dc.identifier.journalChemico-Biological Interactionsen
html.description.abstractThe stabilities of several alkylated nucleic acid components have been examined in the presence of cytosol extracts from a variety of rat tissues. An activity capable of demethylating O6-methyldeoxyguanosine was readily detectable in all tissues examined; arranged in approximate order of decreasing specific activity these are as follows: small intestine, spleen, kidney, lung, liver, skin, heart and brain. The in vitro requirements for the activity derived from liver and the observations that O6-methylguanine and its deoxynucleoside 5'-monophosphate are insensitive to the action of these extracts suggests that this activity may be due to an enzyme which resembles adenosine deaminase. In contrast to the ready degradation of O6-methyldeoxyguanosine the corresponding ethyl derivative was degraded very much more slowly but there was no evidence for other activities against the O4- and O2-methyldeoxythymidines. Similarly, no demethylation of the N-substituted deoxynucleosides, 3-methyldeoxycytidine 3-methldeoxythymidine, 1-methyldeoxyadenosine and 7-methyldeoxyguanosine, was detected.


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