Modulation of target cell susceptibility to human natural killer cells by interferon.
Affiliation
Immunology Division, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, EnglandIssue Date
1980-05-15
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Show full item recordAbstract
The spontaneous cytolytic activity of human peripheral blood lymphocytes in a short-term 51chromium release assay was markedly enhanced by pretreatment with partially purified "Namalva" lymphoblastoid (type I) interferon (IF), provided that the target cells, five lymphoblastoid cell lines of variable natural killer (NK) sensitivity (K562, Molt 4, CCRF/CEM, Raji and Bri8), were not similarly treated with IF. When the targets were pre-exposed to comparable concentrations of IF, their susceptibility to lysis by IF-stimulated or unstimulated effectors was diminished, while exposure of both effectors and targets to IF for the duration of the cytotoxicity assay produced levels of cytotoxicity intermediate between those obtained when either effectors or targets were pretreated. The results indicate that, depending on the experimental design. IF is capable of exerting a protective effect on certain targets, a conclusion supported by competition assays wherein IF-treated cold K562 cells competed less successfully than untreated cold K562 cells for lysis of their radiolabelled counterparts by both IF-stimulated and unstimulated effectors. The data suggest that certain lymphoblastoid targets, in common with effector lymphocytes, possess receptors for IF. Some possible biological implications of the protective interaction of IF with tumour targets, which is antagonistic to that on effector cells, are discussed.Citation
Modulation of target cell susceptibility to human natural killer cells by interferon. 1980, 25 (5):565-72 Int J CancerJournal
International Journal of CancerDOI
10.1002/ijc.2910250504PubMed ID
6154661Type
ArticleLanguage
enISSN
0020-7136ae974a485f413a2113503eed53cd6c53
10.1002/ijc.2910250504