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    The regulation of haemopoiesis in long-term bone marrow cultures: I. role of L-cell CSF.

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    Authors
    Dexter, T Michael
    Shadduck, R
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, England
    Issue Date
    1980-03
    
    Metadata
    Show full item record
    Abstract
    The effects of L-cell conditioned medium which contains granulocyte/macrophage colony stimulating factor (CSF); of highly purified L-cell CSF; and the antiserum directed against L-cell CSF, have been investigated in long-term murine bone marrow cultures. Treatment of cultures with CSF containing conditioned medium led to a rapid decline in haemopoiesis. However, this inhibition of in vitro haemopoiesis is probably caused by materials other than CSF, since the addition of highly purified L-cell CSF had no appreciable effect upon long-term haemopoietic cell proliferation or differentiation. Furthermore, the inhibitory activity of L-cell conditioned medium was not abrogated following neutralization of the CSF activity by CSF antiserum. The direct addition of CSF antiserum did not inhibit granulocyte or macrophage formation. These results suggest that long-term cultures of murine marrow cells may show extensive interactions with stromal cells which are not influenced by exogenous stimulatory or inhibitory factors.
    Citation
    The regulation of haemopoiesis in long-term bone marrow cultures: I. role of L-cell CSF. 1980, 102 (3):279-86 J Cell Physiol
    Journal
    Journal of Cellular Physiology
    URI
    http://hdl.handle.net/10541/189409
    DOI
    10.1002/jcp.1041020302
    PubMed ID
    6967068
    Type
    Article
    Language
    en
    ISSN
    0021-9541
    ae974a485f413a2113503eed53cd6c53
    10.1002/jcp.1041020302
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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