The regulation of haemopoiesis in long-term bone marrow cultures: I. role of L-cell CSF.
Affiliation
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, EnglandIssue Date
1980-03
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The effects of L-cell conditioned medium which contains granulocyte/macrophage colony stimulating factor (CSF); of highly purified L-cell CSF; and the antiserum directed against L-cell CSF, have been investigated in long-term murine bone marrow cultures. Treatment of cultures with CSF containing conditioned medium led to a rapid decline in haemopoiesis. However, this inhibition of in vitro haemopoiesis is probably caused by materials other than CSF, since the addition of highly purified L-cell CSF had no appreciable effect upon long-term haemopoietic cell proliferation or differentiation. Furthermore, the inhibitory activity of L-cell conditioned medium was not abrogated following neutralization of the CSF activity by CSF antiserum. The direct addition of CSF antiserum did not inhibit granulocyte or macrophage formation. These results suggest that long-term cultures of murine marrow cells may show extensive interactions with stromal cells which are not influenced by exogenous stimulatory or inhibitory factors.Citation
The regulation of haemopoiesis in long-term bone marrow cultures: I. role of L-cell CSF. 1980, 102 (3):279-86 J Cell PhysiolJournal
Journal of Cellular PhysiologyDOI
10.1002/jcp.1041020302PubMed ID
6967068Type
ArticleLanguage
enISSN
0021-9541ae974a485f413a2113503eed53cd6c53
10.1002/jcp.1041020302
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