• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Role of apurinic sites in the resistance of methylated oligodeoxyribonucleotides to degradation by spleen exonuclease.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Margison, Geoffrey P
    O'Connor, Peter J
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
    Issue Date
    1975-11
    
    Metadata
    Show full item record
    Abstract
    The effect of introducing methyl groups into DNA substrates was studied by using the spleen exonuclease (EC 3.1.4.1), an enzyme which hydrolyses oligonucleotides in a sequential manner by splitting off 3'-phosphomononucleotides starting from the 5'-hydroxyl terminus. Analyses of oligodeoxyribonucleotide 3'-phosphate substrates after reaction in vitro with dimethyl sulphate demonstrated that the resultant methylation pattern differed from the previously found for native DNA, particularly with respect to the relative amounts of 1- and 3-methyladenine produced. Although after treatment with increasing amounts of dimethyl sulphate the substrate became progressively resistant to degradation by the exonuclease, the methylation products themselves were only partially responsible for the observed inhibition of enzyme activity. The incomplete degradation encountered was apparently due to the presence of apurinic sites, which arose as secondary lesions after the spontaneous release of the labile alkyl purines from the methylated substrate. Inhibition of enzyme activity appeared to be competitive, being characterized by constant values for apparent Vmax, and increased values for apparent Km. the interpretation of this, however, is complicated by the complex nature of the substrate, and these aspects are considered in some detail.
    Citation
    Role of apurinic sites in the resistance of methylated oligodeoxyribonucleotides to degradation by spleen exonuclease. 1975, 151 (2):249-56 Biochem J
    Journal
    The Biochemical Journal
    URI
    http://hdl.handle.net/10541/188452
    PubMed ID
    1218080
    Type
    Article
    Language
    en
    ISSN
    0264-6021
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Homologous recognition and triplex formation promoted by RecA protein between duplex oligonucleotides and single-stranded DNA.
    • Authors: Rao BJ, Chiu SK, Radding CM
    • Issue date: 1993 Jan 20
    • Analysis of substrate specificity of the PaeR7 endonuclease: effect of base methylation on the kinetics of cleavage.
    • Authors: Ghosh SS, Obermiller PS, Kwoh TJ, Gingeras TR
    • Issue date: 1990 Sep 11
    • Molecular enzymology of the EcoRV DNA-(Adenine-N (6))-methyltransferase: kinetics of DNA binding and bending, kinetic mechanism and linear diffusion of the enzyme on DNA.
    • Authors: Gowher H, Jeltsch A
    • Issue date: 2000 Oct 13
    • 3'-Exonuclease resistance of DNA oligodeoxynucleotides containing O6-[4-oxo-4-(3-pyridyl)butyl]guanine.
    • Authors: Park S, Seetharaman M, Ogdie A, Ferguson D, Tretyakova N
    • Issue date: 2003 Apr 1
    • Interaction of the E. coli DNA G:T-mismatch endonuclease (vsr protein) with oligonucleotides containing its target sequence.
    • Authors: Turner DP, Connolly BA
    • Issue date: 2000 Dec 15
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.