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dc.contributor.authorFerreras, Cristina
dc.contributor.authorLancrin, Christophe
dc.contributor.authorLie-A-Ling, Michael
dc.contributor.authorKouskoff, Valerie
dc.contributor.authorLacaud, Georges
dc.date.accessioned2011-10-24T17:28:57Z
dc.date.available2011-10-24T17:28:57Z
dc.date.issued2011-07-21
dc.identifier.citationIdentification and characterization of a novel transcriptional target of RUNX1/AML1 at the onset of hematopoietic development. 2011, 118 (3):594-7 Blooden
dc.identifier.issn1528-0020
dc.identifier.pmid21498670
dc.identifier.doi10.1182/blood-2010-06-294124
dc.identifier.urihttp://hdl.handle.net/10541/146615
dc.description.abstractAlthough the critical requirement for the transcription factor RUNX1/AML1 at the onset of hematopoietic development is well established, little is known about its transcriptional targets at this pivotal stage of blood development. Using microarrays, we identified the uncharacterized gene AI467606 as a gene whose expression level is dramatically reduced in the absence of RUNX1. We further demonstrated by chromatin immunoprecipitation and promoter assay a direct regulation of its transcription by RUNX1. Using a bacterial artificial chromosome transgenic approach, we established that AI467606 is expressed during the development of the hematopoietic system in vivo and in vitro and that its expression is detected within the CD41(+) population and marks definitive hematopoietic potential. Similarly, in the adult mouse, all hematopoietic cell lineages, except mature erythrocytes, express AI467606. Taken together, these findings indicate that AI467606 is a novel transcriptional target of RUNX1/AML1 at the onset of hematopoietic development that is extensively expressed within the hematopoietic system.
dc.language.isoenen
dc.subject.meshAge Factors
dc.subject.meshAnimals
dc.subject.meshBone Marrow
dc.subject.meshCell Lineage
dc.subject.meshCells, Cultured
dc.subject.meshChromosomes, Artificial, Bacterial
dc.subject.meshCore Binding Factor Alpha 2 Subunit
dc.subject.meshGene Expression Regulation, Developmental
dc.subject.meshHematopoiesis
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshMice
dc.subject.meshMolecular Sequence Data
dc.subject.meshPlatelet Membrane Glycoprotein IIb
dc.subject.meshProteins
dc.subject.meshTranscription, Genetic
dc.titleIdentification and characterization of a novel transcriptional target of RUNX1/AML1 at the onset of hematopoietic development.en
dc.typeArticleen
dc.contributor.departmentCancer Research UK Stem Cell Biology Group, Paterson Institute for Cancer Research, University of Manchester, Manchester, United Kingdom.en
dc.identifier.journalBlooden
html.description.abstractAlthough the critical requirement for the transcription factor RUNX1/AML1 at the onset of hematopoietic development is well established, little is known about its transcriptional targets at this pivotal stage of blood development. Using microarrays, we identified the uncharacterized gene AI467606 as a gene whose expression level is dramatically reduced in the absence of RUNX1. We further demonstrated by chromatin immunoprecipitation and promoter assay a direct regulation of its transcription by RUNX1. Using a bacterial artificial chromosome transgenic approach, we established that AI467606 is expressed during the development of the hematopoietic system in vivo and in vitro and that its expression is detected within the CD41(+) population and marks definitive hematopoietic potential. Similarly, in the adult mouse, all hematopoietic cell lineages, except mature erythrocytes, express AI467606. Taken together, these findings indicate that AI467606 is a novel transcriptional target of RUNX1/AML1 at the onset of hematopoietic development that is extensively expressed within the hematopoietic system.


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