Production of stem cell proliferation stimulators and inhibitors by haemopoietic cell suspensions.
dc.contributor.author | Wright, Eric G | |
dc.contributor.author | Lord, Brian I | |
dc.date.accessioned | 2011-10-21T12:21:41Z | |
dc.date.available | 2011-10-21T12:21:41Z | |
dc.date.issued | 1978 | |
dc.identifier.citation | Production of stem cell proliferation stimulators and inhibitors by haemopoietic cell suspensions., 28 (3):156-60 Biomedicine | en |
dc.identifier.issn | 0300-0893 | |
dc.identifier.pmid | 308819 | |
dc.identifier.uri | http://hdl.handle.net/10541/146375 | |
dc.description.abstract | In mice treated with phenylhydrazine haemopoietic spleen colony forming cells (CFU-S) are proliferating rapidly in the bone marrow but not in the spleen. Using such mice we have investigated the production of factors responsible for the control of CFU-S proliferation. When irradiated spleen cells are incubated with non-irradiated bone marrow cells there is a marked fall in the proportion of femoral CFU-S in DNA synthesis. In the converse experiments, rapid triggering of splenic CFU-S is achieved. Both these effects can be eliminated by washing the irradiated cells prior to incubation; they are, however, retained in the supernatant media "conditioned" by these cells. When the washed cells are incubated in fresh medium at 37 degrees C both stimulatory and inhibitory activities reappear but after different incubation periods. The data demonstrate that both proliferation stimulatory and inhibitory factors acting on CFU-S can be produced by the same haemopoietic cell suspension. | |
dc.language.iso | en | en |
dc.subject.mesh | Animals | |
dc.subject.mesh | Bone Marrow | |
dc.subject.mesh | Bone Marrow Cells | |
dc.subject.mesh | Cell Division | |
dc.subject.mesh | Colony-Forming Units Assay | |
dc.subject.mesh | Colony-Stimulating Factors | |
dc.subject.mesh | Hematopoietic Stem Cells | |
dc.subject.mesh | Male | |
dc.subject.mesh | Mice | |
dc.subject.mesh | Mice, Inbred Strains | |
dc.subject.mesh | Phenylhydrazines | |
dc.subject.mesh | Spleen | |
dc.subject.mesh | Time Factors | |
dc.title | Production of stem cell proliferation stimulators and inhibitors by haemopoietic cell suspensions. | en |
dc.type | Article | en |
dc.contributor.department | Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester. | en |
dc.identifier.journal | Biomedicine | en |
html.description.abstract | In mice treated with phenylhydrazine haemopoietic spleen colony forming cells (CFU-S) are proliferating rapidly in the bone marrow but not in the spleen. Using such mice we have investigated the production of factors responsible for the control of CFU-S proliferation. When irradiated spleen cells are incubated with non-irradiated bone marrow cells there is a marked fall in the proportion of femoral CFU-S in DNA synthesis. In the converse experiments, rapid triggering of splenic CFU-S is achieved. Both these effects can be eliminated by washing the irradiated cells prior to incubation; they are, however, retained in the supernatant media "conditioned" by these cells. When the washed cells are incubated in fresh medium at 37 degrees C both stimulatory and inhibitory activities reappear but after different incubation periods. The data demonstrate that both proliferation stimulatory and inhibitory factors acting on CFU-S can be produced by the same haemopoietic cell suspension. |