Show simple item record

dc.contributor.authorWright, Eric G
dc.contributor.authorLord, Brian I
dc.date.accessioned2011-10-21T12:21:41Z
dc.date.available2011-10-21T12:21:41Z
dc.date.issued1978
dc.identifier.citationProduction of stem cell proliferation stimulators and inhibitors by haemopoietic cell suspensions., 28 (3):156-60 Biomedicineen
dc.identifier.issn0300-0893
dc.identifier.pmid308819
dc.identifier.urihttp://hdl.handle.net/10541/146375
dc.description.abstractIn mice treated with phenylhydrazine haemopoietic spleen colony forming cells (CFU-S) are proliferating rapidly in the bone marrow but not in the spleen. Using such mice we have investigated the production of factors responsible for the control of CFU-S proliferation. When irradiated spleen cells are incubated with non-irradiated bone marrow cells there is a marked fall in the proportion of femoral CFU-S in DNA synthesis. In the converse experiments, rapid triggering of splenic CFU-S is achieved. Both these effects can be eliminated by washing the irradiated cells prior to incubation; they are, however, retained in the supernatant media "conditioned" by these cells. When the washed cells are incubated in fresh medium at 37 degrees C both stimulatory and inhibitory activities reappear but after different incubation periods. The data demonstrate that both proliferation stimulatory and inhibitory factors acting on CFU-S can be produced by the same haemopoietic cell suspension.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBone Marrow
dc.subject.meshBone Marrow Cells
dc.subject.meshCell Division
dc.subject.meshColony-Forming Units Assay
dc.subject.meshColony-Stimulating Factors
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMice, Inbred Strains
dc.subject.meshPhenylhydrazines
dc.subject.meshSpleen
dc.subject.meshTime Factors
dc.titleProduction of stem cell proliferation stimulators and inhibitors by haemopoietic cell suspensions.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester.en
dc.identifier.journalBiomedicineen
html.description.abstractIn mice treated with phenylhydrazine haemopoietic spleen colony forming cells (CFU-S) are proliferating rapidly in the bone marrow but not in the spleen. Using such mice we have investigated the production of factors responsible for the control of CFU-S proliferation. When irradiated spleen cells are incubated with non-irradiated bone marrow cells there is a marked fall in the proportion of femoral CFU-S in DNA synthesis. In the converse experiments, rapid triggering of splenic CFU-S is achieved. Both these effects can be eliminated by washing the irradiated cells prior to incubation; they are, however, retained in the supernatant media "conditioned" by these cells. When the washed cells are incubated in fresh medium at 37 degrees C both stimulatory and inhibitory activities reappear but after different incubation periods. The data demonstrate that both proliferation stimulatory and inhibitory factors acting on CFU-S can be produced by the same haemopoietic cell suspension.


This item appears in the following Collection(s)

Show simple item record