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    Fluorescein excitation and emission polarization spectra in living cells: changes during the cell cycle.

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    Authors
    Cercek, L
    Cercek, B
    Ockey, Charles H
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
    Issue Date
    1978-09
    
    Metadata
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    Abstract
    Changes in the fluorescein fluorescence emission and excitation polarization spectra in synchronized cultured S3 fibroblasts at G1, mid-S, and mitosis, as well as in human lymphocytes before and after stimulation with mitogens, were studied. In contrast to those measured in aqueous solutions the emission and excitation polarization spectra in living cells exhibit a wavelength dependence characteristic for the state of the cell cycle. Changes in the temperature and in the amount of intracellular water result in quantitative wavelength-independent changes in the polarization spectra. Possible mechanisms for the qualitative wavelength-dependent changes in the fluorescein emission and excitation polarization spectra during the cell cycle are discussed.
    Citation
    Fluorescein excitation and emission polarization spectra in living cells: changes during the cell cycle. 1978, 23 (3):395-405 Biophys. J.
    Journal
    Biophysical Journal
    URI
    http://hdl.handle.net/10541/146352
    DOI
    10.1016/S0006-3495(78)85458-7
    PubMed ID
    698344
    Type
    Article
    Language
    en
    ISSN
    0006-3495
    ae974a485f413a2113503eed53cd6c53
    10.1016/S0006-3495(78)85458-7
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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