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    Characterisation and separation of human lymphocytes forming mouse red cell rosettes.

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    Authors
    Potter, M R
    Moore, Michael
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
    Issue Date
    1978
    
    Metadata
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    Abstract
    Rosette formation between human lymphocytes and mouse red blood cells (MRBC) was examined as a marker for B lymphocytes and as a method of B lymphocyte separation. A small proportion of lymphocytes formed spontaneous rosettes with MRBC (mean value 6%) and the number was considerably increased by pretreating the lymphocytes with neuraminidase (mean value 16%). Double marker tests demonstrated that lymphocytes forming MRBC rosette were immunoglobulin (Ig) bearing cells, with a high proportion of IgM bearing cells, but not all Ig bearing cells formed MRBC rosettes. Lymphocyte populations enriched with T or B lymphocytes, by SRBC rosette sedimentation and nylon column filtration, gave values for MRBC rosettes consistent with a subpopulation of Ig bearing cells. Separation of MRBC rosette-forming cells gave a relatively poor degree of separation and rerosetting with MRBC produced variable results.
    Citation
    Characterisation and separation of human lymphocytes forming mouse red cell rosettes. 1978, 19 (2-3):125-35 J. Immunol. Methods
    Journal
    Journal of Immunological Methods
    URI
    http://hdl.handle.net/10541/143059
    DOI
    10.1016/0022-1759(78)90173-4
    PubMed ID
    342609
    Type
    Article
    Language
    en
    ISSN
    0022-1759
    ae974a485f413a2113503eed53cd6c53
    10.1016/0022-1759(78)90173-4
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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