Characterisation and separation of human lymphocytes forming mouse red cell rosettes.
AffiliationPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
MetadataShow full item record
AbstractRosette formation between human lymphocytes and mouse red blood cells (MRBC) was examined as a marker for B lymphocytes and as a method of B lymphocyte separation. A small proportion of lymphocytes formed spontaneous rosettes with MRBC (mean value 6%) and the number was considerably increased by pretreating the lymphocytes with neuraminidase (mean value 16%). Double marker tests demonstrated that lymphocytes forming MRBC rosette were immunoglobulin (Ig) bearing cells, with a high proportion of IgM bearing cells, but not all Ig bearing cells formed MRBC rosettes. Lymphocyte populations enriched with T or B lymphocytes, by SRBC rosette sedimentation and nylon column filtration, gave values for MRBC rosettes consistent with a subpopulation of Ig bearing cells. Separation of MRBC rosette-forming cells gave a relatively poor degree of separation and rerosetting with MRBC produced variable results.
CitationCharacterisation and separation of human lymphocytes forming mouse red cell rosettes. 1978, 19 (2-3):125-35 J. Immunol. Methods
JournalJournal of Immunological Methods
- Rosette formation of human lymphoid cells with monkey red blood cells.
- Authors: Pellegrino MA, Ferrone S, Theofilopoulos AN
- Issue date: 1975 Oct
- Binding of mouse red blood cells (MRBC) by human thymocytes: augmentation of rosette formation by phospholipase C treatment.
- Authors: Levy J, Rabinowitz R, Schlesinger M
- Issue date: 1988 Jul
- Allogeneic lymphocyte-dependent antibodies (LDA). Specificity of Fc receptors.
- Authors: Fournier C, Lesavre P, Bach JF
- Issue date: 1977 Mar
- Two small lymphocyte subpopulations in human peripheral blood. I. Purification and surface marker profiles.
- Authors: Hokland M, Hokland P, Heron I
- Issue date: 1978
- Rosette formation by human lymphoid cells with monkey red blood cells.
- Authors: Patel PC, Menezes J, Bourkas A, Dorval G, Rivard GE, Boulay G, Stanley P, Joncas JH
- Issue date: 1978 Apr-Jun