Formation of O2-methylthymine in poly(dA-dT) on methylation with N-methyl-N-nitrosourea and dimethyl sulphate. Evidence that O2-methylthymine does not miscode during DNA synthesis.
| dc.contributor.author | Saffhill, Roy | |
| dc.contributor.author | Abbott, Peter J | |
| dc.date.accessioned | 2011-09-23T16:53:52Z | |
| dc.date.available | 2011-09-23T16:53:52Z | |
| dc.date.issued | 1978-06 | |
| dc.identifier.citation | Formation of O2-methylthymine in poly(dA-dT) on methylation with N-methyl-N-nitrosourea and dimethyl sulphate. Evidence that O2-methylthymine does not miscode during DNA synthesis. 1978, 5 (6):1971-8 Nucleic Acids Res. | en |
| dc.identifier.issn | 0305-1048 | |
| dc.identifier.pmid | 353735 | |
| dc.identifier.doi | 10.1093/nar/5.6.1971 | |
| dc.identifier.uri | http://hdl.handle.net/10541/143057 | |
| dc.description.abstract | The alternating co-polymer has been methylated with either N methyl-N-nitrosourea (MNU) or dimethyl sulphate (DMS) and the levels of the various methylated thymidines (O2-methylthymidine, 3-methylthymidine and O4-methylthymidine) measured. MNU produced all three compounds whereas DMS only produced 3-methylthymidine and O2-methylthymidine at detectable levels. These results have been combined with our earlier results concerning the misincorporation of dGMP with E. coli DNA polymerase using MNU-methylated poly(dA-dT). These results indicate that O2-methylthymidine does not miscode during DNA synthesis. | |
| dc.language.iso | en | en |
| dc.subject.mesh | DNA-Directed DNA Polymerase | |
| dc.subject.mesh | Escherichia coli | |
| dc.subject.mesh | Genetic Code | |
| dc.subject.mesh | Methylation | |
| dc.subject.mesh | Methylnitrosourea | |
| dc.subject.mesh | Nitrosourea Compounds | |
| dc.subject.mesh | Poly dA-dT | |
| dc.subject.mesh | Polydeoxyribonucleotides | |
| dc.subject.mesh | Structure-Activity Relationship | |
| dc.subject.mesh | Thymine | |
| dc.title | Formation of O2-methylthymine in poly(dA-dT) on methylation with N-methyl-N-nitrosourea and dimethyl sulphate. Evidence that O2-methylthymine does not miscode during DNA synthesis. | en |
| dc.type | Article | en |
| dc.contributor.department | Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester | en |
| dc.identifier.journal | Nucleic Acids Research | en |
| html.description.abstract | The alternating co-polymer has been methylated with either N methyl-N-nitrosourea (MNU) or dimethyl sulphate (DMS) and the levels of the various methylated thymidines (O2-methylthymidine, 3-methylthymidine and O4-methylthymidine) measured. MNU produced all three compounds whereas DMS only produced 3-methylthymidine and O2-methylthymidine at detectable levels. These results have been combined with our earlier results concerning the misincorporation of dGMP with E. coli DNA polymerase using MNU-methylated poly(dA-dT). These results indicate that O2-methylthymidine does not miscode during DNA synthesis. |